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Analysis of a bac operon-silenced strain suggests pleiotropic effects of bacilysin in Bacillus subtilis

  • Microbial Physiology and Biochemistry
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Abstract

Bacilysin, as the simplest peptide antibiotic made up of only L-alanine and L-anticapsin, is produced and excreted by Bacillus subtilis under the control of quorum sensing. We analyzed bacilysin-nonproducing strain OGU1 which was obtained by bacA-targeted pMutin T3 insertion into the parental strain genome resulting in a genomic organization (bacA′::lacZ::erm::bacABCDEF) to form an IPTG-inducible bac operon. Although IPTG induction provided 3- to 5-fold increment in the transcription of bac operon genes, no bacilysin activity was detectable in bioassays and inability of the OGU1 to form bacilysin was confirmed by UPLC-mass spectrometry analysis. Phenotypic analyses revealed the deficiencies in OGU1 with respect to colony pigmentation, spore coat proteins, spore resistance and germination, which could be rescued by external addition of bacilysin concentrate into its cultures. 2DE MALDI-TOF/MS and nanoLC-MS/MS were used as complementary approaches to compare cytosolic proteomes of OGU1. 2-DE identified 159 differentially expressed proteins corresponding to 121 distinct ORFs. In nanoLC-MS/MS, 76 proteins were differentially expressed in OGU1. Quantitative transcript analyses of selected genes validated the proteomic findings. Overall, the results pointed to the impact of bacilysin on expression of certain proteins of sporulation and morphogenesis; the members of mother cell compartment-specific σE and σK regulons in particular, quorum sensing and two component-global regulatory systems, peptide transport, stress response as well as CodY- and ScoC-regulated proteins.

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Acknowledgments

This work was supported by the Turkish Scientific and Technical Research Council [the projects TBAG-106T535 and KBAG 116Z351] and partly by Middle East Technical University Scientific Research Funds. We would like to acknowledge Prof. Dörte Becher and Dr. Dirk Albrecht of the Institute of Microbiology, Ernst-Moritz-Arndt-University, Griefswald, Germany and Assoc. Prof. Ömür Çelikbiçak and Dr. Melis Ş. Ekiz of HUNITECH Center of Hacettepe University, Ankara for providing excellent services for MS analyses. We thank our former and present students who provided assistance in certain experiments, and Dr. Sezer Okay for his help in interpretation of comparative genome sequences.

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  1. Department of Biological Sciences/Molecular Biology and Genetics, Middle East Technical University, Ankara, Turkey

    Ozan Ertekin, Meltem Kutnu, Aslı Aras Taşkin, Mustafa Demir & Gülay Özcengiz

  2. Molecular Biology-Biotechnology and Genetics Research Center (MOBGAM) and Molecular Biology and Genetics Department, Istanbul Technical University, Istanbul, Turkey

    Ayten Yazgan Karataş

  3. Faculty of Biology and Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, Germany

    Aslı Aras Taşkin

  4. Biotechnology and Bioengineering, Ecole polytechnique fédérale de Lausanne, Lausanne, Switzerland

    Mustafa Demir

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  1. Ozan Ertekin
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Ertekin, O., Kutnu, M., Taşkin, A.A. et al. Analysis of a bac operon-silenced strain suggests pleiotropic effects of bacilysin in Bacillus subtilis. J Microbiol. 58, 297–313 (2020). https://doi.org/10.1007/s12275-020-9064-0

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  • DOI: https://doi.org/10.1007/s12275-020-9064-0

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