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Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope

  • Microbial Systematics and Evolutionary Microbiology
  • Published:
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Abstract

Two aerobic, Gram-stain-positive, non-motile, non-sporulating coccoid strains, designated ZLJ0423T and ZLJ0321, were isolated from the faeces of Tibetan antelope (Pantholops hodgsonii). Their optimal temperature, NaCl concentration and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ZLJ0423T and ZLJ0321 were very similar to each other (99.8%) and had a sequence similarity of 97.0% with Georgenia satyanarayanai NBRC 107612T and Georgenia subflava CGMCC 1.12782T. Phylogenomic analysis based on 688 core genes indicated that these strains formed a clade with G. satyanarayanai NBRC 107612T and Georgenia wutianyii Z294. The predominant cellular fatty acids were anteiso-C15:0, anteiso-C15:1A and C16:0. The major menaquinone was MK-8(H4). The cell-wall amino acids consisted of alanine, lysine, glycine and aspartic acid, with lysine as the diagnostic diamino acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unidentified lipids formed the polar lipid profile. The DNA G + C content of both isolates was 73.9 mol%. The digital DNA-DNA hybridization value between strains ZLJ0423T and ZLJ0321 was 91.2%, but their values with closely related species and other available type strains of the genus Georgenia were lower than the 70% threshold. On the basis of polyphasic taxonomic data, strains ZLJ0423T and ZLJ0321 represent a novel species within the genus Georgenia, for which the name Georgenia faecalis sp. nov. is proposed. The type strain is ZLJ0423T (= CGMCC 1.13681T = JCM 33470T).

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Acknowledgments

This work was supported by grants from the National Science and Technology Major Project of China (2018ZX1071-2001-007), National Key R&D Program of China (2018YFC-1200102), Sanming Project of Medicine in Shenzhen (SZSM-201811071), and Research Units of Discovery of Unknown Bacteria and Function (2018RU010), Chinese Academy of Medical Sciences, the Key Research and Development Program of Hainan Province (ZDYF2019149, ZDYF2017163); the Science and Technology Innovation Project of Medical Health in Sanya (2019YW20).

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Authors and Affiliations

  1. Central & Clinical Laboratory of Sanya People’s Hospital, Sanya, Hainan, 572000, P. R. China

    Xiaoxia Wang, Licheng Wang, Limei Han, Sha Li, Huan Li, Xiaoying Fu, Hai Chen & Xiong Zhu

  2. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, 102206, P. R. China

    Xiaoxia Wang & Jing Yang

  3. Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai, 201508, P. R. China

    Jing Yang

  4. Research Units of Discovery of Unknown Bacteria and function, Chinese Academy of Medical Sciences, Beijing, 100730, P. R. China

    Jing Yang

  5. Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, P. R. China

    Yuyuan Huang

  6. Shaanxi Institute of Zoology, Xi’an, 710032, P. R. China

    Xiaomin Wu

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  1. Xiaoxia Wang
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Correspondence to Xiong Zhu.

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Conflict of Interest

The authors declare that there are no conflicts of interest.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains ZLJ0423T and ZLJ0321 are MH136554 and MK156345 respectively. The GenBank/EMBL/DDBJ accession numbers for the genome sequences of strains ZLJ0423T and ZLJ0321 are CP033325 and RHDW00000000, respectively.

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Wang, X., Yang, J., Huang, Y. et al. Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope. J Microbiol. 58, 734–740 (2020). https://doi.org/10.1007/s12275-020-0060-1

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