Skip to main content
SpringerLink
Log in

Mesorhizobium denitrificans sp. nov., a novel denitrifying bacterium isolated from sludge

  • Microbial Systematics and Evolutionary Microbiology
  • Published:
Journal of Microbiology Aims and scope Submit manuscript

Abstract

A Gram-stain-negative, non-spore-forming, facultative, rod-shaped bacterium (designated LA-28T) was isolated from a sludge sample from a wastewater treatment plant in Hanam city, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain LA-28T clustered with species of the genus Mesorhizobium and appeared closely related to M. jarvisii LMG 28313T (96.8%), M. waimense ICMP 19557T (96.7%), and M. huakuii LMG 14107T (96.7%). Growth occurs at 18–40°C on R2A medium in the presence of 1–4% NaCl (w/v) and at pH 6–8. The DNA G+C content was 61.2 mol%, and the predominant quinone was ubiquinone-10 (Q-10). The major cellular fatty acids (> 5%) were C16:0, C19:0ω8c cyclo, C18:1ω7c 11-methyl, and C18:1ω7c and/or C18:1ω6c (summed feature 8). Major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidyl-N-methylethanolamine (PME), and phosphatidylcholine (PC). Physiological and biochemical characteristics indicated that strain LA-28T represents a novel species of the genus Mesorhizobium, for which the name Mesorhizobium denitrificans sp. nov. is proposed. The type strain is LA-28T (= KACC 19675T = LMG 30806T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Atlas, R.M. 1993. Handbook of microbiological media. CRC Press, Boca Raton, Florida, USA.

    Google Scholar 

  • Buck, J.D. 1982. Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl. Environ. Microbiol. 44, 992–993.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Cappuccino, J.G. and Sherman, N. 2002. Microbiology. a laboratory manual, 6th ed. Pearson Education, Inc., California, USA.

    Google Scholar 

  • Chen, W.X., Li, G.S., Qi, Y.L., Wang, E.T., Yuan, H.L., and Li, J.L. 1991. Rhizobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int. J. Syst. Bacteriol. 41, 275–280.

    Article  Google Scholar 

  • De Meyer, S.E., Tan, H.W., Andrews, M., Heenan, P.B., and Willems, A. 2016. Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules. Int. J. Syst. Evol. Microbiol. 66, 786–795.

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783–791.

    Article  PubMed  Google Scholar 

  • Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416.

    Article  Google Scholar 

  • Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.

    CAS  Google Scholar 

  • Hiraishi, A., Ueda, Y., Ishihara, J., and Mori, T. 1996. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J. Gen. Appl. Microbiol. 42, 457–469.

    Article  CAS  Google Scholar 

  • Jarvis, B.D.W., Van Berkum, P., Chen, W.X., Nour, S.M., Fernandez, M.P., Cleyet-Marel, J.C., and Gillis, M. 1997. Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int. J. Syst. Bacteriol. 47, 895–898.

    Article  Google Scholar 

  • Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press, Cambridge, UK.

    Book  Google Scholar 

  • Lane, D.J. 1991. 16S/23S rRNA sequencing. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic acid techniques in bacterial systematics. Wiley, New York, USA.

    Google Scholar 

  • Martinez-Hidalgo, P., Ramirez-Bahena, M.H., Flores-Felix, J.D., Igual, J.M., Sanjuan, J., Leon-Barrios, M., Peix, A., and Velazquez, E. 2016. Reclassification of strains MAFF 303099T and R7A into Mesorhizobium japonicum sp. nov. Int. J. Syst. Evol. Microbiol. 66, 4936–4941.

    Article  CAS  PubMed  Google Scholar 

  • Mesbah, M., Premachandran, U., and Whitman, W.B. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.

    Article  CAS  Google Scholar 

  • Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.

    Article  CAS  Google Scholar 

  • Moore, D.D. and Dowhan, D. 1995. Preparation and analysis of DNA, pp. 2–11. In Ausubel, F.W., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds.), Current protocols in molecular biology, Wiley, New York, USA.

    Google Scholar 

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.

    CAS  PubMed  Google Scholar 

  • Sasser, M. 1990. Identification of bacteria through fatty acid analysis, pp. 199–204. In Klement, Z., Rudolph, K., and Sands, D.C. (eds.), Methods in phytobacteriology, Akademiai Kaido, Budapest, Hungary.

    Google Scholar 

  • Siddiqi, M.Z., Shah, S., Choi, K.D., Kim, S.Y., and Im, W.T. 2018. Mesorhizobium hankyongi sp. nov. isolated from soil of ginseng cultivating field. Curr. Microbiol. 75, 1453–1459.

    Article  CAS  PubMed  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725–2729.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins., D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Velázquez, E., Igual, J.M., Willems, A., Fernández, M.P., Muñoz, E., Mateos, P.F., Abril, A., Toro, N., Normand, P., Cervantes, E., et al. 2001. Mesorhizobium chacoense sp. nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina). Int. J. Syst. Evol. Microbiol. 51, 1011–1021.

    Article  PubMed  Google Scholar 

  • Yuan, C.G., Jiang, Z., Xiao, M., Zhou, E.M., Kim, C.J., Hozzein, W.N., Park, D.J., Zhi, X.Y., and Li, W.J. 2016. Mesorhizobium sediminum sp. nov., isolated from deep-sea sediment. Int. J. Syst. Evol. Microbiol. 66, 4797–4802.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Hankyong National University, Anseong, 17579, Republic of Korea

    Muhammad Zubair Siddiqi, Dae-Cheol Kim, Young-Woo Lee & Wan-Taek Im

  2. AceEMzyme Co., Ltd., Academic Industry Cooperation, Anseong, 17579, Republic of Korea

    Muhammad Zubair Siddiqi, Gyumin Choi & Wan-Taek Im

  3. M-ChET, KIOCA program, Hankyong National University, Anseong, 17579, Republic of Korea

    Ngo Thi Phuong Thao

  4. Department of Food Science & Bio Technology, Shinansan University, Ansan, 15435, Republic of Korea

    Sang Young Kim & Ji-Hyang Wee

Authors
  1. Muhammad Zubair Siddiqi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ngo Thi Phuong Thao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gyumin Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dae-Cheol Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Young-Woo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sang Young Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ji-Hyang Wee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wan-Taek Im
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wan-Taek Im.

Additional information

Supplemental material for this article may be found at https://doi.org/www.springerlink.com/content/120956.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Siddiqi, M.Z., Thao, N.T.P., Choi, G. et al. Mesorhizobium denitrificans sp. nov., a novel denitrifying bacterium isolated from sludge. J Microbiol. 57, 238–242 (2019). https://doi.org/10.1007/s12275-019-8590-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-019-8590-0

Keywords

Search

Navigation