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Paenibacillus seodonensis sp. nov., isolated from a plant of the genus Campanula

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Abstract

Strain DCT-19T, representing a Gram-stain-positive, rodshaped, aerobic bacterium, was isolated from a native plant belonging to the genus Campanula on Dokdo, the Republic of Korea. Comparative analysis of the 16S rRNA gene sequence showed that this strain was closely related to Paenibacillus amylolyticus NRRL NRS-290T (98.6%, 16S rRNA gene sequence similarity), Paenibacillus tundrae A10bT (98.1%), and Paenibacillus xylanexedens NRRL B-51090T (97.6%). DNADNA hybridization indicated that this strain had relatively low levels of DNA-DNA relatedness with P. amylolyticus NRRL NRS-290T (30.0%), P. xylanexedens NRRL B-51090T (29.0%), and P. tundrae A10bT (24.5%). Additionally, the genomic DNA G + C content of DCT-19T was 44.8%. The isolated strain grew at pH 6.0–8.0 (optimum, pH 7.0), 0–4% (w/v) NaCl (optimum, 0%), and a temperature of 15–45°C (optimum 25–30°C). The sole respiratory quinone in the strain was menaquinone-7, and the predominant fatty acids were C15:0 anteiso, C16:0 iso, and C16:0. In addition, the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Based on its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain DCT-19T is proposed as a novel species in the genus Paenibacillus, for which the name Paenibacillus seodonensis sp. nov. is proposed (=KCTC 43009T =LMG 30888T). The type strain of Paenibacillus seodonensis is DCT-19T.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Biological Resources Utilization Department, National Institute of Biological Resources, Incheon, 22689, Republic of Korea

    Myung-Suk Kang

  2. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea

    Ki-Eun Lee & Eun-Young Lee

  3. Department of Biology, Jeju National University, Jeju, 63243, Republic of Korea

    Soo-Je Park

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  1. Myung-Suk Kang
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  2. Ki-Eun Lee
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  4. Soo-Je Park
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Correspondence to Soo-Je Park.

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Kang, MS., Lee, KE., Lee, EY. et al. Paenibacillus seodonensis sp. nov., isolated from a plant of the genus Campanula. J Microbiol. 56, 874–879 (2018). https://doi.org/10.1007/s12275-018-8455-y

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  • DOI: https://doi.org/10.1007/s12275-018-8455-y

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