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Spirosoma lituiforme sp. nov., isolated from soil

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A Gram-staining-negative, non-motile, curved rod-shaped, aerobic bacterium, designated S1-2-4T, was isolated from soil in Jeollabuk-do province, Republic of Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain S1-2-4T was a member of the family Cytophagaceae and most closely related to ‘Spirosoma radiotolerans’ DG5A (97.2%), Spirosoma fluviale MSd3T (96.4%), and Spirosoma linguale DSM 74T (96.3%). The genomic DNA G + C content of strain S1-2-4T was 49.7 mol%. The major fatty acids were summed feature 3 (C16:1ω7c/C16:1ω6c), C16:1ω5c, and C16:0, and the major polar lipid was phosphatidylethanolamine. MK-7 was the predominant respiratory quinone. Phenotypic and chemotaxonomic data supported the affiliation of strain S1-2-4T with the genus Spirosoma. DNA-DNA hybridization between strain S1-2-4T and ‘Spirosoma radiotolerans’ showed relatively low DNA-DNA relatedness (31%). Strain S1-2-4T could be distinguished from its closest phylogenetic neighbors based on its phenotypic, genotypic, and chemotaxonomic features. Therefore, strain S1-2-4T represents a novel member of the genus Spirosoma, for which the name Spirosoma lituiforme sp. nov. is proposed. The type strain is S1-2-4T (= KCTC 52724T = JCM 32128T).

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Weilan Li, Seung-Yeol Lee, Sangkyu Park, Leonid N. Ten & Hee-Young Jung

  2. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea

    Byung-Oh Kim

  3. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Hee-Young Jung

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  1. Weilan Li
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  2. Seung-Yeol Lee
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  5. Leonid N. Ten
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Correspondence to Hee-Young Jung.

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Li, W., Lee, SY., Park, S. et al. Spirosoma lituiforme sp. nov., isolated from soil. J Microbiol. 55, 856–861 (2017). https://doi.org/10.1007/s12275-017-7255-0

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  • DOI: https://doi.org/10.1007/s12275-017-7255-0

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