Abstract
Methanol (MeOH) extract of the aerial parts of Dendropanax morbifera (Araliaceae) has demonstrated a significant dose-dependent inhibitory effect on the RANKL-induced differentiation of bone marrow-derived macrophages to osteoclasts. Bioassay-guided fractionation of the extract resulted in the isolation of a novel diacetylene carboxylic acid (1), together with a known diacetylenic compounds (2) as phytochemicals to strongly inhibit the osteoclast differentiation. The chemical structure of 1 was determined by spectroscopic analyses as (9Z,16S)-16-O-acetyl-9,17-octadecadiene-12,14-diynoic acid, that is acetyl derivative of 2. Two diacetylenic components of D. morbifera, 1 and 2 exhibited a dose-dependent inhibitory effect on the RANKL-induced formation of tartrate-resistant acid phosphatase-positive multinucleated cells with IC50 values of 2.4 and 3.1 μM, respectively. Seven other known components (3–9) were also isolated from the extract: dendropanoxide (3), friedelin (4), epifriedelanol (5), α-amyrin (6), β-amyrin (7), β-sitosterol (8), and stigmasterol (9). The significant anti-osteoclastogenic activities of 3, 4, 5, and 7 were first reported in this study.
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Acknowledgements
This work was supported by Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration in 2015 (C0277170) and by the Ministry of Science, ICT, and Future Planning of Korea (SI-1606).
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Kim, E.H., Jo, C.S., Ryu, S.Y. et al. Anti-osteoclastogenic diacetylenic components of Dendropanax morbifera. Arch. Pharm. Res. 41, 506–512 (2018). https://doi.org/10.1007/s12272-018-1033-3
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DOI: https://doi.org/10.1007/s12272-018-1033-3