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Self-organized nanoparticles based on chitosan-folic acid and dextran succinate-doxorubicin conjugates for drug targeting

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Abstract

Folic acid-decorated self-organized nanoparticles were fabricated to target folate receptor of cancer cells. Doxorubicin (DOX) was conjugated with carboxyl group of dextran backbone using succinic anhydride (DexSU-DOX). DOX-loaded self-organized nanoparticles were prepared by complexation with folic acid-grafted chitosan (ChitoFA) and DexSU-DOX. Nanoparticles in the aqueous environment have spherical shapes with average size less than 100 nm and their sizes were increased by coated with ChitoPEG or ChitoFA. At cell culture study with KB cells, ChitoFA coated nanoparticles (FADex NP) revealed folate-receptor mediated endocytosis to cancer cells and cell viability was significantly changed by folate receptor targeting. Tumor xenograft model of KB cells also showed similar results, i.e. FAdex NP efficiently inhibited growth of tumor compared to the treatment group with blocking of folate receptor. These results indicated that DOX-loaded nanoparticles of FADex NP are promising vehicle for anticancer drug targeting.

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Authors and Affiliations

  1. Department of Oriental Medicine Materials, Dongsin University, Naju, 520-714, Republic of Korea

    Kyung Dong Lee

  2. Department of Medical Science, Pusan National University Medical School, Yangsan, Gyeonnam, 626-770, Republic of Korea

    Seon-Hee Choi

  3. Faculty of Life and Environmental Science, Shimane University, Matsue, Japan

    Da Hye Kim

  4. Department of Beauty Stylist, Yeonsung University, Anyang, Gyeonggido, 430-749, Republic of Korea

    Hye-Young Lee

  5. Grassland and Forages Research Center, National Institute of Animal Science, Rural Development Administration, Cheonan, Chungnam, 331-808, Republic of Korea

    Ki-Choon Choi

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  1. Kyung Dong Lee
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  2. Seon-Hee Choi
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Correspondence to Ki-Choon Choi.

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Lee, K.D., Choi, SH., Kim, D.H. et al. Self-organized nanoparticles based on chitosan-folic acid and dextran succinate-doxorubicin conjugates for drug targeting. Arch. Pharm. Res. 37, 1546–1553 (2014). https://doi.org/10.1007/s12272-014-0489-z

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  • DOI: https://doi.org/10.1007/s12272-014-0489-z

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