Skip to main content
SpringerLink
Log in

Proteine mit ausreichend Häm-Kofaktor beladen

  • Wissenschaft · Methoden
  • Rekombinante Proteine
  • Published:
BIOspektrum Aims and scope

Abstract

Heme proteins play an important role in several biological processes. For their functional analysis, recombinant proteins are widely used. However, the saturation of the proteins with the heme cofactor is often limited by the availability of heme inside the host cell. Several different methods to improve the recombinant production of heme proteins have been established, the most promising ones are being summarized here.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Mills M, Payne SM (1995) Genetics and regulation of heme iron transport in Shigella dysenteriae and detection of an analogous system in Escherichia coli 0157:H7.1 Bacteriol 177:3004–3009

    Article  CAS  Google Scholar 

  2. Fiege K, Querebillo CI, Hildebrandt P et al. (2018) Improved method for the incorporation of heme cofactors into recombinant proteins using Escherichia coli Nissle 1917 Biochemistry 57:2747–2755

    CAS  PubMed  Google Scholar 

  3. Woodard SI, Dailey HA (1995) Regulation of heme biosynthesis in Escherichia coli. Arch Biochem Biophys 316:110–115

    Article  CAS  PubMed  Google Scholar 

  4. Wu C, Zhang I, Abu-Soud H et al. (1996) High-level expression of mouse inducible nitric oxide synthase in Escherichia coli requires coexpression with calmodulin. Biochem Biophys Res Commun 222:439–444

    Article  CAS  PubMed  Google Scholar 

  5. Chen H, Jiang P (2019) Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phyco-biliprotein. Microb Cell Fact 18:58

    Article  PubMed  PubMed Central  Google Scholar 

  6. Varnado CL, Goodwin DC (2004) System for the expression of recombinant hemoproteins in Escherichia coli. Protein Expr Purif 35:76–83

    Article  CAS  PubMed  Google Scholar 

  7. Sonnenborn U, Schulze I (2009) The non-pathogenic Escherichia coli strain Nissle 1917 - features of a versatile probiotic. Microb Ecol Health Dis 21:122–158

    Article  CAS  Google Scholar 

  8. Grozdanov L, Raasch C, Schulze I et al. (2004) Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917. J Bacteriol:1 5432–5441

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fachbereich Biologie, Abteilung Mikrobiologie Technische Universität Kaiserslautern, Paul-Ehrlich-Straße 23, D-67663, Kaiserslautern, Deutschland

    Kerstin Fiege &  Nicole Frankenberg-Dinkel

Authors
  1. Kerstin Fiege
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nicole Frankenberg-Dinkel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nicole Frankenberg-Dinkel.

Additional information

Kerstin Fiege Jahrgang 1988. 2008–2014 Bachelor- und Masterstudium Biologie (Schwerpunkt Mikrobiologie und Infektionsbiologie) an der TU Braunschweig. 2013 Laborpraktikum bei Prof. Dr. C. von Wachenfeldt an der Lund University, Schweden. 2014–2019 Promotion bei Prof. Dr. N. Frankenberg-Dinkel an der Universität Bochum und der TU Kaiserslautern.

Nicole Frankenberg-Dinkel Jahrgang 1971. 1990–1996 Biologiestudium an den Universitäten Regensburg und Boulder, Colorado, USA. 1996–1999 Promotion bei Prof. Dr. D. Jahn an der Universität Freiburg. 1999–2002 Postdoc bei Prof. Dr. J. Lagarias an der University of California, Davis, USA. 2003–2005 Emmy-Noether-Nachwuchsgruppe an der TU Braunschweig. 2005 Habilitation. 2006–2014 W2-Professorin für Physiologie der Mikroorganismen an der Universität Bochum. Seit 2014 W3-Professorin für Mikrobiologie an der TU Kaiserslautern.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fiege, K., Frankenberg-Dinkel, N. Proteine mit ausreichend Häm-Kofaktor beladen. Biospektrum 25, 510–512 (2019). https://doi.org/10.1007/s12268-019-1087-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12268-019-1087-x

Search

Navigation