Abstract
Influenza viruses remain an imminent threat for animal and public health. Rapid and reliable diagnosis of acute infections are at the roots of meaningful control and prevention measures. Sensitive and highly specific reverse transcription real-time PCR (RT-qPCR) assays have revolutionized the diagnostic toolbox. RT-qPCRs for subtyping and pathotype characterization of animal influenza viruses provide advantages in individual diagnosis and for long term monitoring programs.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literatur
Arya M, Shergill IS, Williamson M et al. (2005) Basic principles of real-time quantitative PCR. Exp Rev Mol Diagn 5:209–219
Cheung TK, Poon LL (2007) Biology of influenza A virus. In: Biology of Emerging Viruses: Sars, Avian and Human Influenza, Metapneumovirus, Nipah, West Nile, and Ross River Virus. Lal SK (Hrsg). Annals of the New York Academy of Sciences 1102:1–25
Su S, Fu XL, Li GR et al. (2017) Novel influenza D virus: epidemiology, pathology, evolution and biological characteristics. Virulence 8:1580–1591
Paules C, Subbarao K (2017) Influenza. Lancet 390:697–708
Franca MS, Brown JD (2014) Influenza pathobiology and pathogenesis in avian species. Curr Top Microbiol Immunol 385:221–242
Rajao DS, Anderson TK, Gauger PC et al. (2014) Pathogenesis and vaccination of influenza A virus in swine. Curr Top Microbiol Immunol 385:307–326
Petrova VN, Russell CA (2018) The evolution of seasonal influenza viruses. Nat Rev Microbiol 16:47
Short KR, Richard M, Verhagen JH et al. (2015) One health, multiple challenges: the inter-species transmission of influenza A virus. One Health 1:1–13
Spackman E, Suarez DL (2008) Avian influenza virus. Type A influenza virus detection and quantitation by realtime RT-PCR. Methods Mol Biol 436:19–26
Globig A, Staubach C, Sauter-Louis C et al. (2018) Highly pathogenic avian influenza H5N8 clade 2.3.4.4b in Germany in 2016/2017. Front Vet Sci 4:240
Graaf A, Beer M, Harder T (2017) Real-time reverse transcription PCR-based sequencing-independent pathotyping of Eurasian avian influenza A viruses of subtype H7. Virol J 14:137
Naguib MM, Graaf A, Fortin A et al. (2017) Novel realtime PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017. Eurosurveillance 22:15–26
Anderson TK, Macken CA, Lewis NS et al. (2016) A phy-logeny-based global nomenclature system and automated annotation tool for H1 hemagglutinin genes from swine influenza A viruse. mSphere 1:e00275–16
Henritzi D, Hoffmann B, Wacheck S et al. (2019) A newly developed tetraplex real-time RT-PCR for simultaneous screening of influenza virus types A, B, C and D. Influenza Other Respir Viruses 13:71–82
Henritzi D, Zhao N, Starick E et al. (2016) Rapid detection and subtyping of European swine influenza viruses in porcine clinical samples by haemagglutinin- and neuramini-dase-specific tetra- and triplex real-time RT-PCRs. Influenza Other Respir Viruses 10:504–517
Hoffmann B, Hoffmann D, Henritzi D et al. (2016) Riems influenza a typing array (RITA): an RT-qPCR-based low density array for subtyping avian and mammalian influenza a viruses. Sci Rep 6:27211
Author information
Authors and Affiliations
Corresponding author
Additional information
Dinah Henritzi 2005–2013 Biologiestudium (Diplom) an der Rheinischen Friedrich-Wilhelms-Universitüt Bonn. 2015–2018 Promotion und anschließend Postdoc am Friedrich-Loeffler-Institut, Insel Riems, unter der Leitung von Prof. Dr. T. Harder.
Annika Graaf 2010–2016 Veterinärmedizinstudium an der Ludwig-Maximilians-Universität München. 2016–2019 Promotion und Postdoc am Friedrich-Loeffler-Institut, Insel Riems, unter der Leitung von Prof. Dr. T. Harder.
Timm Harder 1983–1989 Tiermedizinstudium an der Tierärztlichen Hochschule Hannover; dort 1989–1993 Promotion und Postdoc. 1994–1996 Postdoc an der Erasmus Universiteit, Rotterdam; Postdoc an der Christian-Albrechts-Universität Kiel (Habilitation und apl. Professur). Seit 2006 am Friedrich-Loeffler-Institut, Insel Riems, Leiter des Nationalen Referenzlabors für Aviäre Influenza und Experte für Influenzainfektionen der Tiere bei der Welttiergesundheitsorganisation (O. I. E.).
Rights and permissions
About this article
Cite this article
Henritzi, D., Graaf, A. & Harder, T. Spezifisch — sicher — schnell: Nachweis von Influenzaviren. Biospektrum 25, 278–281 (2019). https://doi.org/10.1007/s12268-019-1046-6
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12268-019-1046-6