Abstract
Site-specific proteolytic processing is an irreversible post-translational protein modification with essential regulatory functions. Dedicated methods enable proteome-wide characterization of differentially processed proteoforms based on their distinct protease-generated N termini. Exemplary profiling of murine glomeruli revealed processed forms of proteins with important functions in the renal filtration barrier. Altered processing was observed in cellular and animal models of glomerular disease.
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Fatih Demir 2001–2006 Biologiestudium an der Universität Heidelberg. 2007–2010 Promotion in Pflanzenphysiologie an der Universität Würzburg. 2011–2014 Postdoc am Institut für Neuround Sinnesphysiologie des Uniklinikums Düsseldorf. 2014–2015 Postdoc in der Neurologischen Klinik des Uniklinikums Düsseldorf. Seit 2015 Postdoc am Forschungszentrum Jülich.
Andreas Perrar 2009–2011 Ausbildung zum biologisch-technischen Assistenten. 2011–2014 Bachelorstudium Biologie an der Universität zu Köln. 2014–2016 Masterstudium Plant Sciences an der Universität Bonn. Seit 2017 Doktorand am Forschungszentrum Jülich und an der Universität zu Köln.
Pitter Huesgen 1998–2002 Chemiestudium an den Universitäten Marburg und Stockholm, Schweden. 2003–2007 Promotion in Biologie und 2007–2008 Postdoc an der Universität Konstanz. 2008–2014 Postdoc an der University of British Columbia in Vancouver, Kanada. Seit 2014 Teamleiter am Forschungszentrum Jülich. Seit 2019 Professor für Proteindynamik und Proteolyse an der Universität zu Köln.
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Demir, F., Perrar, A. & Huesgen, P.F. Das maßgeschneiderte Proteom: Proteinmodifikation durch Proteolyse. Biospektrum 25, 41–44 (2019). https://doi.org/10.1007/s12268-019-1003-4
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DOI: https://doi.org/10.1007/s12268-019-1003-4