Abstract
The macrocyclic hydroxamate siderophore avaroferrin of Shewanella algae inhibits ferric iron dependent swarming motility of Vibrio alginolyticus. Investigating the biosynthesis of avaroferrin and related siderophores demonstrates the importance of the substrate pool for product formation and reveals an unprecedented flexibility in the substrate range of the responsible synthetases. Exploiting the promiscuity of these enzymes allowed to generate a broad spectrum of 15 different ring-size engineered siderophores some of which also inhibit Vibrio’s swarming behavior.
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Sina Rütschlin2010–2015 Chemiestudium an der Universität Konstanz. Seit 2015 Doktorandin in der Arbeitsgruppe von Dr. T. Böttcher mit einem KoRS-CB-Stipendium.
Thomas Böttcher2003–2006 Chemie- und Biochemiestudium an der LMU München, dort 2006–2009 Promotion bei Prof. Dr. S. A. Sieber. 2010 Gründer und Projektleiter der AVIRU GmbH für anti-infektive Wirkstoffentwicklung. 2011–2014 Postdoc bei Prof. Dr. J. Clardy an der Harvard Medical School in Boston, USA. Seit 2014 unabhängiger Emmy-Noether-Forschungsgruppenleiter an der Universität Konstanz.
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Rütschlin, S., Böttcher, T. Eine vielseitige Enzymklasse für die Synthese ringförmiger Siderophore. Biospektrum 24, 484–487 (2018). https://doi.org/10.1007/s12268-018-0950-5
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DOI: https://doi.org/10.1007/s12268-018-0950-5