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Neue Spektren in der Durchflusszytometrie

  • Wissenschaft · Special: Durchflusszytometrie
  • Multispektraldurchflusszytometrie
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Abstract

Flow cytometry (FC) is one of the most powerful tools for cell analysis. Recent technical and analytical advances have the potential to revolutionize this technique and may even expand its power for clinical diagnostics. Here, we provide an overview on three of these innovations, namely hyperspectral and multispectral FC, fluorescence minus one controls used for de novo population identification, and FC standardization using a pulsed LED source.

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Literatur

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Author information

Authors and Affiliations

  1. Deutsches Rheuma-Forschungszentrum Berlin (DRFZ), Leibniz-Gemeinschaft, Berlin, Deutschland

    Claudia Giesecke & Toralf Kaiser

  2. Berlin-Brandenburg School for Regenerative Therapies (BSRT), Berlin, Deutschland

    Claudia Giesecke

  3. Deutsches Rheuma-Forschungszentrum Berlin (DRFZ) Flow Cytometry Core Facility, Charitéplatz 1, D-10117, Berlin, Deutschland

    Claudia Giesecke & Toralf Kaiser

Authors
  1. Claudia Giesecke
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  2. Toralf Kaiser
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Corresponding authors

Correspondence to Claudia Giesecke or Toralf Kaiser.

Additional information

Claudia Giesecke Jahrgang 1984. 2005–2008 Biochemiestudium (Bachelor) an der Universität Potsdam. 2008–2011 Masterstudium Molekulare Medizin an der Charité Berlin. 2010–2015 wissenschaftliche Mitarbeiterin und Promotion in der Medizinischen Klinik mit Schwerpunkt Rheumatologie und Klinische Immunologie, Charité Berlin. Seit 2016 Postdoc an der Berlin-Brandenburg School for Regenerative Therapies in der Guppe Zellbiologie am Deutschen Rheuma-Forschungszentrum (DRFZ) Berlin.

Toralf Kaiser Jahrgang 1968. 1989–1997 Studium der Technischen Physik in Jena und Berlin. 1997–2000 Operator für Durchflusszytometrie am Max-Planck-Institut für Infektionsbiologie. Seit 2000 technischer Leiter der Flow Cytometry Core Facility (FCCF) am Deutschen Rheuma-Forschungszentrum (DRFZ) Berlin.

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Giesecke, C., Kaiser, T. Neue Spektren in der Durchflusszytometrie. Biospektrum 23, 658–661 (2017). https://doi.org/10.1007/s12268-017-0854-9

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  • DOI: https://doi.org/10.1007/s12268-017-0854-9

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