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MicroRNA-29b-3p Targets SPARC Gene to Protect Cardiocytes against Autophagy and Apoptosis in Hypoxic-Induced H9c2 Cells

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Abstract

MicroRNAs participate in the regulation of abnormal cardiomyocyte apoptosis and autophagy, which leads to heart failure (HF). Lower miR-29b-3p levels were found in HF patients in this study. However, the role of miR-29b-3p in the molecular pathogenesis of HF remains unclear. Hypoxia-stimulated H9c2 cells were used an in vitro model of HF. It was found that hypoxia stimulation decreased the miR-29b-3p expression and enhanced cell apoptosis and autophagy response in H9c2 cells. While the effects of hypoxia on cell apoptosis and autophagy were reversed by miR-29b-3p transfection, especially 100 nM. The secreted protein acidic and rich in cysteine (SPARC), predicted as a direct target of miR-29b-3p, aggravated the hypoxia-induced cells apoptosis, autophagy, and TGFβ1/Smad3 activation. While the changes were dramatically reversed by miR-29b-3p. Taken together, our data suggest that miR-29b-3p plays an important role in the progression of HF through targeting SPARC and regulating TGFβ1/Smad3 pathway.

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Acknowledgements

The authors would like to thank the members of the Second Department of Cardiology, Xinxiang Central Hospital for participating and providing blood samples in this study.

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Authors and Affiliations

  1. Second Department of Cardiology, Xinxiang Central Hospital, Xinxiang, Henan, China

    Shu Zhou, Dazhou Lei, Faqin Bu, Hongqiang Han, Shucai Zhao & Yan Wang

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  1. Shu Zhou
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  2. Dazhou Lei
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  3. Faqin Bu
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  4. Hongqiang Han
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  5. Shucai Zhao
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  6. Yan Wang
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Correspondence to Yan Wang.

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The author declares that they have no conflicts of interest.

Human Subjects/Informed Consent Statement

The present study was approved by the Ethics Committee of Xinxiang Central Hospital, and written informed consent was obtained from the patients and healthy donors.

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No animal studies were carried out by the authors for this article.

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Associate Editor Joost Sluijter oversaw the review of this article

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Zhou, S., Lei, D., Bu, F. et al. MicroRNA-29b-3p Targets SPARC Gene to Protect Cardiocytes against Autophagy and Apoptosis in Hypoxic-Induced H9c2 Cells. J. of Cardiovasc. Trans. Res. 12, 358–365 (2019). https://doi.org/10.1007/s12265-018-9858-1

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  • DOI: https://doi.org/10.1007/s12265-018-9858-1

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