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Synchrotron Radiation-Based Three-Dimensional Visualization of Angioarchitectural Remodeling in Hippocampus of Epileptic Rats

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Abstract

Characterizing the three-dimensional (3D) morphological alterations of microvessels under both normal and seizure conditions is crucial for a better understanding of epilepsy. However, conventional imaging techniques cannot detect microvessels on micron/sub-micron scales without angiography. In this study, synchrotron radiation (SR)-based X-ray in-line phase-contrast imaging (ILPCI) and quantitative 3D characterization were used to acquire high-resolution, high-contrast images of rat brain tissue under both normal and seizure conditions. The number of blood microvessels was markedly increased on days 1 and 14, but decreased on day 60 after seizures. The surface area, diameter distribution, mean tortuosity, and number of bifurcations and network segments also showed similar trends. These pathological changes were confirmed by histological tests. Thus, SR-based ILPCI provides systematic and detailed views of cerebrovascular anatomy at the micron level without using contrast-enhancing agents. This holds considerable promise for better diagnosis and understanding of the pathogenesis and development of epilepsy.

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Acknowledgements

This work was completed at the BL13W1 beamline of the Shanghai Synchrotron Radiation Facility (SSRF) in China and was supported by Key Research Project of the Ministry of Science and Technology of China (2016YFC0904400), the National Natural Science Foundation of China (81501025 & 81671299), the Natural Science Foundation of Hunan Province (2016JJ3174), and the Science and Technology Department Funds of Hunan Province Key Project (2016JC2057). We would like to thank Prof. Tiqiao Xiao and other staff at the BL13W1 station of SSRF for their kind assistance during the experiments.

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  1. Pan Gu, Zi-Hao Xu and Yu-Ze Cao have contributed equally to this work.

Authors and Affiliations

  1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China

    Pan Gu, Zi-Hao Xu, Yu-Ze Cao, Qian-Fang Deng, Zhuo-Hui Chen, Xin-Hang Hu, Hui Wang, Li-Zhi Li, Shi-Xin Liu, Hui Ding, Shu-Peng Shi, Hong-Lei Li, Bo Xiao & Meng-Qi Zhang

  2. School of Information Science and Engineering, Central South University, Changsha, 410008, China

    Sheng-Hui Liao

  3. State Key Laboratory of Drug Research, CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China

    Xian-Zhen Yin

  4. Department of Breast Surgery, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, 410008, China

    Zhuo-Lu Wang

  5. Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201800, China

    Ti-Qiao Xiao

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  1. Pan Gu
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Gu, P., Xu, ZH., Cao, YZ. et al. Synchrotron Radiation-Based Three-Dimensional Visualization of Angioarchitectural Remodeling in Hippocampus of Epileptic Rats. Neurosci. Bull. 36, 333–345 (2020). https://doi.org/10.1007/s12264-019-00450-0

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