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The Effect of Extractum Cepae, Heparin Sodium, and Allantoin in Experimental Peritoneal Adhesion

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Abstract

The purpose of the study is to investigate the effects of extractum cepae (EC), heparin sodium (HS), and allantoin (AA) on postoperative peritoneal adhesion (PPA) prevention. Forty-eight rats were allocated into six groups (n = 8). PPA was performed by Harris method. In group 1, no additional procedure was done. Abdominal cavity was washed with normal saline in group 2, EC in group 3, HS in group 4, AA in group 5, and EC-HS-AA mixture (EHA-m) in group 6. On the 21st postoperative day, PPA was scored with Nair adhesion score and examined for fibrosis histopathologically and hydroxy-proline levels (OHP) biochemically. Macroscopically, PPA was decreased in groups 2, 4, and 6 compared to groups 1, 3, and 5 (p < 0.05) and decreased in group 2 compared to other groups (p < 0.05). Histopathologically, fibrosis was decreased in groups 2, 4, and 6 compared to other groups and decreased in group 2 compared to other groups (p < 0.05). Tissue OHP level was decreased in groups 2, 3, 4, and 5 compared to groups 1 and 6 (p < 0.05). Tissue OHP level was decreased in group 6 compared to group 1, but the difference was not statistically significant. PPA formation decreases with normal saline, HS and EHA-m, whereas increases with EC and AA both macroscopically and microscopically. Although HS and EHA-m seem to have adhesion prevention effect, washing the intestines with saline seems to be more effective.

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Acknowledgements

This study was accepted as the master thesis of Ismail Ozmen, M.D. (Kırıkkale University, Faculty of Medicine, and Department of Pediatric Surgery). The thesis advisors are Boybeyi-Turer O and Gunal YD.

This study was presented as an oral presentation in the 33rd Congress of National Pediatric Surgery Association, Antalya, Turkey, 2015.

Funding

This study was supported by Kırıkkale University Scientific Research Council (2014/81) for only purchasing laboratory animal and other materials stated in the methods.

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Authors and Affiliations

  1. Faculty of Medicine Department of Pediatric Surgery, Kırıkkale University, Kırıkkale, Turkey

    Ismail Ozmen, Yasemin Dere Gunal & Mustafa Kemal Aslan

  2. Faculty of Medicine, Department of Pathology, Kırıkkale University, Kırıkkale, Turkey

    Pinar Atasoy

  3. Faculty of Medicine, Department of Biochemistry, Kırıkkale University, Kırıkkale, Turkey

    Ucler Kisa

  4. Faculty of Science, Department of Statistics, Kırıkkale University, Kırıkkale, Turkey

    Serap Yorubulut

  5. Faculty of Medicine, Department of Pediatric Surgery, Hacettepe University, Ankara, Turkey

    Ozlem Boybeyi-Turer

Authors
  1. Ismail Ozmen
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  2. Yasemin Dere Gunal
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  3. Pinar Atasoy
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  4. Ucler Kisa
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  5. Serap Yorubulut
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  6. Mustafa Kemal Aslan
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  7. Ozlem Boybeyi-Turer
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Contributions

The authors contributed to the study as follows: (i) conception and design: I Ozmen and O Boybeyi-Turer; (ii) materials and experiments: I Ozmen and Y Dere Gunal; (iii) analysis and interpretation: P Atasoy, U Kısa, and S Yörübulut; (iv) writer and supervision: O Boybeyi-Turer; and (v) critical review: Y Dere Gunal and O Boybeyi-Turer.

Corresponding author

Correspondence to Ozlem Boybeyi-Turer.

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Ethical Approval

All experiments were performed in accordance with the ethical standards of the institution at which the studies were conducted (Kirikkale Local Ethical Committee, Date and Number of Permission: 30/01/2014–14/02-22).

Conflict of Interest

The authors, Ozmen I, Dere Gunal Y, Atasoy P, Kisa U, Yorubulut S, Aslan MK, and Boybeyi-Turer, declare that they have no any conflict of interest.

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Ozmen, I., Gunal, Y.D., Atasoy, P. et al. The Effect of Extractum Cepae, Heparin Sodium, and Allantoin in Experimental Peritoneal Adhesion. Indian J Surg 82, 892–898 (2020). https://doi.org/10.1007/s12262-020-02176-0

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  • DOI: https://doi.org/10.1007/s12262-020-02176-0

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