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Development and Evaluation of the Airtight, Minimal-Invasive, and Fast Device Harvesting Adipose Tissue for Autologous Fat Grafting

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Abstract

The present study was undertaken to develop the device that can be used to harvest adipose tissue for fat grafting with an airtight, minimal-invasive, and fast approach. The abdominal liposuction was performed in six women under local tumescent anesthesia. Fat from left abdomen was harvested by the classical 20-ml-syringe technique while fat from right abdomen was harvested by a reformative vacuum-assisted suction device (negative pressure, 25 to 35 kPa). This device includes a 1-L sterile canister, a cannula with 2.5 mm inner diameter, a conventional liposuction machine, and a one-way valve connected between the canister and the liposuction machine. The operative time of harvesting 200-ml fat grafts was recorded and calculated. The collected adipose tissue was used for adipose-derived stem cells (ADSCs) extraction and culture, glucose transport test (GTT), and bacteriological culture. First, we found that the operative time of harvesting 200-ml adipose tissue using 20-ml-syringe aspiration technique was 71.3 ± 4.3 min compared with 30 ± 3.5 min by the new device (p < 0.05). The sizes of the fat grafts obtained from both methods were similar. Second, the viability of harvested adipose tissue tested through GTT showed no significant difference between two harvesting methods (p > 0.05). Additionally, the samples harvested from both methods showed no bacterial growth. The results from the XTT test revealed that there was no significant difference on ADSCs growth between two methods (p > 0.05). In conclusion, the results support that the innovative device for adipose tissue harvesting is minimal-invasive, fast, and can prevent the fat grafts from contamination.

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Funding

This work was supported by the National Nature and Science Foundation, China (81372065 and 81871563), the Major Project of Guangzhou Municipal Science and Technology Bureau (201300000091 and 201508020253), the Fundamental Research Funds for the Central Universities (21619350), and the Foster Research Fund of The First Affiliated Hospital of Jinan University (2017306).

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Author notes

  1. Hai-Ling Huang and Xuan Liao contributed equally to this work.

Authors and Affiliations

  1. Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Innovative Technology Research Institute of Tissue Repair and Regeneration, Key Laboratory of Regenerative Medicine, Ministry of Education, Guangzhou, 510630, Guangdong, China

    Hai-Ling Huang, Xuan Liao, Hong-Wei Liu, Wen-Li She, Bo Xie, Li-Ling Xiao, Guang-Hui Xie & Yi-Wei Huang

  2. Department of Medical Equipment, The First Affiliated Hospital of Jinan University, Guangzhou, 510630, Guangdong Province, People’s Republic of China

    Wei-Guang Ou

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  1. Hai-Ling Huang
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  2. Xuan Liao
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Correspondence to Hong-Wei Liu.

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Ethics Approval and Consent to Participate

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent for participation in the study or use of their tissue was obtained from all participants. This article does not contain any studies with animals performed by any of the authors.

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Huang, HL., Liao, X., Liu, HW. et al. Development and Evaluation of the Airtight, Minimal-Invasive, and Fast Device Harvesting Adipose Tissue for Autologous Fat Grafting. Indian J Surg 82, 545–550 (2020). https://doi.org/10.1007/s12262-019-02036-6

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  • DOI: https://doi.org/10.1007/s12262-019-02036-6

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