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Regulatory Role of SFN Gene in Hepatocellular Carcinoma and Its Mechanism

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Abstract

Purpose: This study aims to explore the differential expression of SFN gene and its regulatory role in different hepatocarcinoma cells, and the impact on hepatocarcinoma. Materials and Methods: High and low SFN expression cells were screened by qRT-PCR and western blotting methods. SFN over expression and interference vectors were constructed. Cell viability was detected by CCK8 kit, cell cycle and apoptosis were detected by flow cytometry. Cell invasion and migration were detected. CCNB1 and CDK1 expression levels were detected by qRT-PCR and Western blotting methods. Results: The high SFN expression BEL7402 cells and the low SFN expression Hep3B cells were screened from Hep3B, HepG2, and BEL7402 cells. The activity of Hep3B cells overexpression vector SFN-pcDNA3.1(+) decreased and apoptosis increased, the ratio of G0/G1 decreased and the ratio of S phase increased. The activity of BEL7402 cells transfected with SFN siRNA decreased and apoptosis increased, the ratio of G0/G1 decreased and the ratio of G2/M increased. Interference and overexpression vectors have little effect on the invasion and migration of the two cells. The expression of CDK1 in Hep3B cells decreased significantly, the expression of CDK1 and CCNB1 in BEL7402 cells increased significantly. Conclusions: The differentially expressed SFN gene can regulate the growth of the two hepatocarcinoma cells, high expression of SFN gene can inhibit their growth. The mechanism may be achieved by regulating CCNB1 and CDK1 expression.

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Acknowledgement

This study was supported by research project of Guangxi Health Committee (Z20200814).

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  1. Equal contributors

Authors and Affiliations

  1. Clinical Laboratory, Guigang People’s Hospital, Guigang, Guangxi, 537100, China

    Ying Hui, Hao Zeng, Yi Feng, Wenzhou Qin, Peisheng Chen, Lifang Huang, Wenfu Zhong, Liwen Lin & Hui Lv

  2. Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China

    Xue Qin

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  1. Ying Hui
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  2. Hao Zeng
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  3. Yi Feng
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Contributions

Conceived and designed the experiments: Ying Hui and Xue Qin; Execution of experiments: Hao Zeng, Ying Hui and Wenzhou Qin; Data analysis: Peisheng Chen and Ying Hui; Discussion of results: Hao Zeng, Lifang Huang, Wenfu Zhong and Xue Qin; Wrote the manuscript: Liwen Lin, Hui Lv and Hao Zeng, Critical reading of manuscript: Ying Hui, Yi Feng, and Xue Qin.

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Correspondence to Ying Hui or Xue Qin.

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Hui, Y., Zeng, H., Feng, Y. et al. Regulatory Role of SFN Gene in Hepatocellular Carcinoma and Its Mechanism. Biotechnol Bioproc E 26, 375–383 (2021). https://doi.org/10.1007/s12257-020-0292-2

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  • DOI: https://doi.org/10.1007/s12257-020-0292-2

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