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Biodegradation of toxic organic compounds using a newly isolated Bacillus sp. CYR2

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Abstract

The objective of this study was to isolate a new bacterium and investigate its ability for degradation of various toxic organic compounds. Based on 16S rRNA gene sequence and phylogenetic analysis, the isolated strain was identified as Bacillus sp. CYR2. Degradation of various toxic compounds and growth of CYR2 strain were evaluated with 2 and 4% inoculum sizes. All the experiments were conducted for 6 days, flasks were incubated at 30oC under 180 rpm. Among the 2 and 4% inoculum sizes, bacteria showed highest growth and toxic compounds degradation at 4% inoculum size. Especially, compared to 2% inoculum size, growth of the strain CYR2 at 4% inoculum size was increased by 15.1 folds with 4-secondarybutylphenol, 9.1 folds with phenol, and 5.4 folds with 4-tertiary-butylphenol. Strain CYR2 at 4% inoculum size showed highest removal of phenol (84 ± 5%), followed by 4-tertiary-butylphenol (66 ± 3%), 4-secondary-butylphenol (63 ± 5%) and 4-nonylphenol (57 ± 6%). Compared with 2% inoculum size, degradation ability of strain CYR2 with 4% inoculum size was enhanced by 3.45 times with 4-tertiary-octylphenol, and 2.53 times with 4-tertiarybutylphenol. Our results indicated that the newly isolated Bacillus sp. CYR2 can be used for in situ bioremediation of phenol and alkylphenols contaminated water.

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Authors and Affiliations

  1. Department of Applied Sciences, College of Environmental Technology, Muroran Institute of Technology, Muroran, 050-8585, Japan

    M. Venkateswar Reddy & Young-Cheol Chang

  2. Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto-shi, Kyoto, 606-8501, Japan

    Yuka Yajima

  3. Biotechnology Laboratory, B-K Company Ltd R & D, Gunsan, 54008, Korea

    DuBok Choi

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  1. M. Venkateswar Reddy
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  2. Yuka Yajima
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  3. DuBok Choi
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  4. Young-Cheol Chang
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Correspondence to DuBok Choi or Young-Cheol Chang.

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Reddy, M.V., Yajima, Y., Choi, D. et al. Biodegradation of toxic organic compounds using a newly isolated Bacillus sp. CYR2. Biotechnol Bioproc E 22, 339–346 (2017). https://doi.org/10.1007/s12257-017-0117-0

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  • DOI: https://doi.org/10.1007/s12257-017-0117-0

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