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Bacterial cellulose-chitosan composite hydrogel beads for enzyme immobilization

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Abstract

In this work, we report the preparation of bacterial cellulose (BC)-chitosan composite hydrogel beads by co-dissolution of BC and chitosan in 1-ethyl-3-methylimidazolium acetate and subsequent reconstitution with distilled water. The BC-chitosan hydrogel beads were used as enzyme supports for immobilizing Candida rugosa lipase by physical adsorption and covalent cross-linking. BC-chitosan hydrogel beads immobilized lipase more efficiently than microcrystalline cellulose (MCC)-chitosan hydrogel beads. The amount of protein adsorbed onto BCchitosan beads was 3.9 times higher than that adsorbed onto MCC-chitosan beads, and the catalytic activity of lipase was 1.9 times higher on the BC-chitosan beads. The lipase showed the highest thermal and operational stability when covalently cross-linked on BC-chitosan hydrogel beads. The half-life time of the lipase cross-linked on BC-chitosan bead at 60°C was 22.7 times higher than that of free lipase. Owing to their inherent biocompatibility and biodegradability, the BC-chitosan composite hydrogel beads described here could be used to immobilize proteins for various biomedical, environmental, and biocatalytic applications.

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Authors and Affiliations

  1. Department of Microbial Engineering, Konkuk University, Seoul, 05025, Korea

    Hyun Jung Kim & Sang Hyun Lee

  2. Alvogen Korea, Seoul, 07326, Korea

    Ju Nam Jin

  3. Texas A&M AGRILIFE Research & Extension Center, Texas A&M University, Stephenville, TX, 76401, USA

    Eunsung Kan

  4. Office of Sponsored Projects, Tarleton State University, Stephenville, TX, 76401, USA

    Eunsung Kan

  5. Urban Agriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, 441-440, Korea

    Kwang Jin Kim

Authors
  1. Hyun Jung Kim
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  2. Ju Nam Jin
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  3. Eunsung Kan
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  4. Kwang Jin Kim
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  5. Sang Hyun Lee
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Correspondence to Sang Hyun Lee.

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Kim, H.J., Jin, J.N., Kan, E. et al. Bacterial cellulose-chitosan composite hydrogel beads for enzyme immobilization. Biotechnol Bioproc E 22, 89–94 (2017). https://doi.org/10.1007/s12257-016-0381-4

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  • DOI: https://doi.org/10.1007/s12257-016-0381-4

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