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A solid-phase screening method for identification of glycan-binding cells

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Abstract

Glycan immobilization and epitope recognition on a solid support is useful for functional glycoproteomics and analysis of protein-protein interactions. However, the lack of high-throughput experimental methods for the examination of cell-glycan interactions means that identifying glycan-binding cells remains a challenge. Herein, we describe a tool which enables the screening of glycanbinding cells that specifically recognize the glycan epitopes released from the membrane glycoproteins of cells and tissues. Biotinylated pig kidney N-glycans were immobilized on streptavidin-conjugated solid supports and then incubated with human immune cell lines. U937 cells discriminated between the α-galactose (α-Gal) and non-Gal pig N-glycans on the solid support. This screening strategy could aid exploration of cell-protein or cell-cell interactions.

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Authors and Affiliations

  1. Department of Chemical Engineering, Soongsil University, Seoul, 156-743, Korea

    Yoon-Woo Kim, Kyoung-Jin Kim, Cheol-Hwan Hwang & Yun-Gon Kim

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  1. Yoon-Woo Kim
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  2. Kyoung-Jin Kim
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  4. Yun-Gon Kim
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Correspondence to Yun-Gon Kim.

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Kim, YW., Kim, KJ., Hwang, CH. et al. A solid-phase screening method for identification of glycan-binding cells. Biotechnol Bioproc E 20, 366–372 (2015). https://doi.org/10.1007/s12257-014-0662-8

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  • DOI: https://doi.org/10.1007/s12257-014-0662-8

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