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Experimental study and kinetic modeling of cometabolic degradation of phenol and p-nitrophenol by loofa-immobilized Ralstonia eutropha

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Abstract

In the present study, phenol-adapted cells of Ralstonia eutropha were used to degrade p-nitrophenol (PNP) in the presence of phenol. PNP at initial concentrations ranging from 5 to 15 mg/L was degraded almost completely by free cells of R. eutropha. The use of loofa-immobilized cells increased the complete removal of PNP up to 30 mg/L. Kinetic data for PNP biodegradation by immobilized cells of R. eutropha best fitted the Haldane model. The kinetic parameters were k s = 0.0006 (mg PNP/mg biomass.h), K s = 8.83 (mg/L) and K i = 30.77 (mg/L). The degradation pathways of PNP through the metabolites, 4-nitro-catechol (4-NC) and hydroquinone (HQ), were investigated using HPLC.

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Authors and Affiliations

  1. Chemical Engineering Department, Amirkabir University of Technology, Hafez Ave., Tehran, 15875-4413, Iran

    Mohammad Maleki, Mahdi Motamedi, Mahsa Sedighi & Farzaneh Vahabzadeh

  2. Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, 14115-143, Iran

    Seyed Morteza Zamir

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  1. Mohammad Maleki
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  2. Mahdi Motamedi
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  3. Mahsa Sedighi
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  4. Seyed Morteza Zamir
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  5. Farzaneh Vahabzadeh
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Correspondence to Seyed Morteza Zamir.

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Maleki, M., Motamedi, M., Sedighi, M. et al. Experimental study and kinetic modeling of cometabolic degradation of phenol and p-nitrophenol by loofa-immobilized Ralstonia eutropha . Biotechnol Bioproc E 20, 124–130 (2015). https://doi.org/10.1007/s12257-014-0593-4

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  • DOI: https://doi.org/10.1007/s12257-014-0593-4

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