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Dictyopteris undulata extract induces apoptosis in human colon cancer cells

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Abstract

The present study investigated the cytotoxic and apoptotic effects of an ethanol extract derived from the marine brown alga Dictyopteris undulata against human colon adenocarcinoma cells. The Dictyopteris undulata extract (DUE) showed cytotoxic activity against SW480 cells in a dose-dependent manner, with 50% inhibition of cell viability at a concentration of 40 μg/mL. DUE also induced programmed cell death in SW480 cells, as evidenced by apoptotic body formation, DNA fragmentation, an increase in the population of apoptotic sub-G1 phase cells, and mitochondrial membrane depolarization. Moreover, DUE significantly modulated the expression of apoptosisassociated proteins, resulting in a decrease in B cell lymphoma-2 expression and an increase in Bcl-2-associated X protein expression, as well as the activation of caspase-9 and caspase-3. Furthermore, DUE showed apoptotic cell death in two other colon cancer cell lines, SNU407 and HT29. These observations suggest that DUE may prove useful as a therapeutic agent for the attenuation of colon cancer.

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Authors and Affiliations

  1. School of Medicine, Jeju National University, Jeju, 690-756, Korea

    Areum Daseul Kim, Kyoung Ah Kang, Mei Jing Piao, Ki Cheon Kim, Jian Zheng, Cheng Wen Yao, Ji Won Cha, Chang Lim Hyun, Sun Jin Boo, Soo Young Na & Jin Won Hyun

  2. Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju, 690-756, Korea

    Areum Daseul Kim & Nam Ho Lee

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  1. Areum Daseul Kim
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Correspondence to Soo Young Na or Jin Won Hyun.

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Kim, A.D., Kang, K.A., Piao, M.J. et al. Dictyopteris undulata extract induces apoptosis in human colon cancer cells. Biotechnol Bioproc E 19, 419–425 (2014). https://doi.org/10.1007/s12257-014-0200-8

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  • DOI: https://doi.org/10.1007/s12257-014-0200-8

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