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Phenotypic Characterization of Porcine IFNγ-Producing Lymphocytes in Porcine Reproductive and Respiratory Syndrome Virus Vaccinated and Challenged Pigs

  • Research Article
  • Published:
Virologica Sinica

Abstract

Porcine reproductive and respiratory syndrome (PRRS) continues to be one of the most important swine diseases worldwide. Interferon-γ (IFNγ)-mediated type I cell-mediated immune response plays an important role in protection from, and clearance of, PRRS virus (PRRSV). Several lymphocyte subsets including T-helper, CTLs, Th/memory cells, and γδ T lymphocytes were previously reported to produce IFNγ during PRRSV infection. However, the proportion and phenotypic characterization of these IFNγ-secreting lymphocytes have not been explored. In this study, IFNγ producted by different lymphocyte subsets was assessed by multi-color flow cytometry after vaccination with PRRSV modified live vaccine (PRRSV-MLV) and challenge with homogeneous or heterogeneous PRRSV. The results showed that T-helper cells were the major IFNγ-secreting cell population after PRRSV-MLV vaccination and PRRSV challenge. Additionally, the proportion of IFNγ producing Th/memory cells and γδ T cells increased after PRRSV challenge. This difference was accounted for an enhanced ability to produce IFNγ in Th/memory cells and an enlarged quantity of γδ T cells. The results presented here could contribute to our understanding of the roles of IFNγ in protective immunity against PRRSV infection and may be useful for assessment of cell-mediated immunity in vaccine tests.

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References

  • Chand RJ (2012) Pathogenesis of porcine reproductive and respiratory syndrome virus. Curr Opin Virol 2:256–263

    Article  CAS  PubMed  Google Scholar 

  • Costers S, Lefebvre DJ, Goddeeris B, Delputte PL, Nauwynck HJ (2009) Functional impairment of PRRSV-specific peripheral CD3+CD8high cells. Vet Res 40:46

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • De Bruin TG, Van Rooij EM, De Visser YE, Bianchi AT (2000) Cytolytic function for pseudorabies virus-stimulated porcine CD4+CD8dull+ lymphocytes. Viral Immunol 13:511–520

    Article  PubMed  Google Scholar 

  • Gerner W (2009) Porcine T lymphocytes and NK cells—an update. Dev Comp Immunol 33:310–320

    Article  CAS  PubMed  Google Scholar 

  • Guo B, Lager KM, Henningson JN, Miller LC, Schlink SN, Kappes MA, Kehrli ME Jr, Brockmeier SL, Nicholson TL, Yang HC, Faaberg KS (2013) Experimental infection of United States swine with a Chinese highly pathogenic strain of porcine reproductive and respiratory syndrome virus. Virology 435:372–384

    Article  CAS  PubMed  Google Scholar 

  • Jiang Y, Xiao S, Fang L, Yu X, Song Y, Niu C, Chen H (2006) DNA vaccines co-expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV) display enhanced immunogenicity. Vaccine 24:2869–2879

    Article  CAS  PubMed  Google Scholar 

  • Keane NM, Roberts SG, Almeida CA, Krishnan T, Chopra A, Demaine E, Laird R, Tschochner M, Carlson JM, Mallal S, Heckerman D, James I, John M (2012) High-avidity, high-IFNgamma-producing CD8 T-cell responses following immune selection during HIV-1 infection. Immunol Cell Biol 90:224–234

    Article  CAS  PubMed  Google Scholar 

  • Kimman TG, Cornelissen LA, Moormann RJ, Rebel JM, Stockhofe-Zurwieden N (2009) Challenges for porcine reproductive and respiratory syndrome virus (PRRSV) vaccinology. Vaccine 27:3704–3718

    Article  CAS  PubMed  Google Scholar 

  • Li X, Galliher-Beckley A, Huang H, Sun X, Shi J (2013) Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus. Vaccine 41:4508–4515

    Article  CAS  Google Scholar 

  • Li X, Galliher-Beckley A, Pappan L, Trible B, Kerrigan M, Beck A, Hesse R, Blecha F, Nietfeld JC, Rowland RR, Shi J (2014) Comparison of host immune responses to homologous and heterologous type II porcine reproductive and respiratory syndrome virus (PRRSV) challenge in vaccinated and unvaccinated pigs. Biomed Res Int 2014:416727

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lunney JK, Fritz ER, Reecy JM, Kuhar D, Prucnal E, Molina R, Christopher-Hennings J, Zimmerman J, Rowland RR (2010) Interleukin-8, interleukin-1beta, and interferon-gamma levels are linked to PRRS virus clearance. Viral Immunol 23:127–134

    Article  CAS  PubMed  Google Scholar 

  • Murtaugh MP, Genzow M (2011) Immunological solutions for treatment and prevention of porcine reproductive and respiratory syndrome (PRRS). Vaccine 29:8192–8204

    Article  PubMed  Google Scholar 

  • Riganti C, Massaia M, Davey MS, Eberl M (2012) Human gammadelta T-cell responses in infection and immunotherapy: common mechanisms, common mediators? Eur J Immunol 42:1668–1676

    Article  CAS  PubMed  Google Scholar 

  • Rodriguez-Carreno MP, Lopez-Fuertes L, Revilla C, Ezquerra A, Alonso F, Dominguez J (2002) Phenotypic characterization of porcine IFN-gamma-producing lymphocytes by flow cytometry. J Immunol Methods 259:171–179

    Article  CAS  PubMed  Google Scholar 

  • Sinkora M, Butler JE (2009) The ontogeny of the porcine immune system. Dev Comp Immunol 33:273–283

    Article  CAS  PubMed  Google Scholar 

  • Sun J, Zhao H, Li N, Sun Y, Xi Z, Zhou Y, Wang Y, Qi Q, Lu C, Qiu H (2008a) Immune responses induced by the suicidal DNA vaccines co-expressing the GP5 protein of PRRSV and the E2 protein of CSFV in mice. Sheng Wu Gong Cheng Xue Bao 24:1714–1722 (in Chinese)

    Article  CAS  PubMed  Google Scholar 

  • Sun N, Liu D, Chen H, Liu X, Meng F, Zhang X, Xie S, Li X, Wu Z (2008b) Localization, expression change in PRRSV infection and association analysis of the porcine TAP1 gene. Int J Biol Sci 8:49–58

    Article  Google Scholar 

  • Toyoda M, Ge S, Suviolahti E, Pichurin P, Shin B, Pao A, Vo A, Deer N, Aguiluz A, Karasyov A, Jordan SC (2012) IFNgamma production by NK cells from HLA-sensitized patients after in vitro exposure to allo-antigens. Transpl Immunol 26:107–112

    Article  CAS  PubMed  Google Scholar 

  • Xiao Z, Batista L, Dee S, Halbur P, Murtaugh MP (2004) The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of virus load. J Virol 78:5923–5933

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang X, Wang X, Mu L, Ding Z (2012) Immune responses in pigs induced by recombinant DNA vaccine co-expressing Swine IL-18 and membrane protein of porcine reproductive and respiratory syndrome virus. Int J Mol Sci 13:5715–5728

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This work was supported by Grant from The National Natural Science Foundation of China (NSFC, Grant No. 31490601), National Key Research and Development Program (Grant No. 2016YFD0500703), Major Science and Technology Projects in Henan Province (Grant No. 171100110200), and Luoyang Heluo Talent Plan (Dr. Kegong Tian).

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Author notes

  1. Xiangdong Li, Zengyang Pei and Yilin Bai have contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China

    Kegong Tian

  2. National Research Center for Veterinary Medicine, High-Tech District, Luoyang, 471003, China

    Xiangdong Li & Kegong Tian

  3. Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, 66506, USA

    Xiangdong Li, Zengyang Pei, Lihua Wang & Jishu Shi

  4. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China

    Yilin Bai

  5. Department of Veterinary Medicine, College of Animal Science, Zhejiang University, Hangzhou, 310058, China

    Zengyang Pei

  6. OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, Beijing, 100125, China

    Kegong Tian

Authors
  1. Xiangdong Li
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  2. Zengyang Pei
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  3. Yilin Bai
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  4. Lihua Wang
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  5. Jishu Shi
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Contributions

JS and KT design the study. XL, ZP, YB and LW performed the experiments. XL analyzed the data and drafted the manuscript. XL, JS and KT wrote the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Jishu Shi or Kegong Tian.

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The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

The whole study was approved by the Animal Ethics Committee of National Research Center for Veterinary Medicine in China (Permit No. NVC-2017-018). All institutional and national guidelines for the care and use of laboratory animals were followed.

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Li, X., Pei, Z., Bai, Y. et al. Phenotypic Characterization of Porcine IFNγ-Producing Lymphocytes in Porcine Reproductive and Respiratory Syndrome Virus Vaccinated and Challenged Pigs. Virol. Sin. 33, 524–530 (2018). https://doi.org/10.1007/s12250-018-0073-7

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  • DOI: https://doi.org/10.1007/s12250-018-0073-7

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