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Association of single nucleotide polymorphism rs2065955 of the filaggrin gene with susceptibility to Epstein-Barr virus-associated gastric carcinoma and EBV-negative gastric carcinoma

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Virologica Sinica

Abstract

The relationship between the Filaggrin gene (FLG) rs2065955 polymorphism and susceptibility to Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma (EBVnGC) was investigated in Shandong Province, China. We detected the FLG rs2065955 genotype and allele distribution by using PCR and restriction fragment length polymorphism (RFLP) in 64 EBVaGC, 82 EBVnGC, and 111 normal control samples. Immunohistochemistry was used to detect the level of FLG protein in 35 EBVaGC and 51 EBVnGC tumor tissues. Compared with normal controls, the genotype CC and allele C of FLG rs2065955 showed higher frequency in EBVaGC and EBVnGC. There was no significant difference between EBVaGC and EBVnGC in allele distribution of FLG rs2065955, but the genotype CC was found more frequently in EBVaGC than in EBVnGC. The risk of developing either EBVaGC or EBVnGC in genotype CC was higher than in other genotypes. Furthermore, genotype CC of FLG rs2065955 may contribute more to the risk of developing EBVaGC than EBVnGC. There was no significant difference in the expression level of FLG protein between EBVaGC and EBVnGC. In conclusion, the FLG rs2065955 polymorphism was significantly related to gastric carcinoma. Allele C of FLG rs2065955 could be a risk factor for EBVaGC or EBVnGC, while genotype CC of FLG rs2065955 was especially associated with EBVaGC.

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Authors and Affiliations

  1. Department of Medical Microbiology, Qingdao University Medical College, Qingdao, 266021, China

    Xiaojing Kuang, Zhenzhen Zhao, Danrui Zhao, Song Liu & Bing Luo

  2. Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China

    Lingling Sun

  3. Department of Blood Transfusion, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China

    Shuzhen Liu

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  1. Xiaojing Kuang
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  2. Lingling Sun
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  3. Shuzhen Liu
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  4. Zhenzhen Zhao
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  5. Danrui Zhao
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  6. Song Liu
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  7. Bing Luo
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Correspondence to Bing Luo.

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ORCID: 0000-0002-5675-8510

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Kuang, X., Sun, L., Liu, S. et al. Association of single nucleotide polymorphism rs2065955 of the filaggrin gene with susceptibility to Epstein-Barr virus-associated gastric carcinoma and EBV-negative gastric carcinoma. Virol. Sin. 31, 306–313 (2016). https://doi.org/10.1007/s12250-016-3721-9

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