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A KASP Marker for the Potato Late Blight Resistance Gene RB/Rpi-blb1

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Abstract

The disease late blight is a threat to potato production worldwide, making genetic resistance an important target for breeding. The resistance gene RB/Rpi-blb1 is effective against most strains of the causal pathogen, Phytophthora infestans. Until now, potato breeders have utilized a Sequence Characterized Amplified Region (SCAR) marker to screen for RB. Our objective was to design and validate a Kompetitive Allele Specific PCR (KASP) marker, which has advantages for high-throughput screening. First, the accuracy of the SCAR marker was confirmed in two segregating tetraploid populations. Then, using whole genome sequencing data for two RB-positive segregants and a diverse set of 23 RB-negative varieties, a SNP in the 5’ untranslated (UTR) region was identified as unique to RB. The KASP marker based on this SNP, which had 100% accuracy in the cultivated diversity panel, was used to generate diploid breeding lines containing RB. The KASP marker is publicly available for others to utilize.

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Data Availability

SNP array and phenotype data are provided as Supplemental Files S1 and S2, respectively. Whole-genome sequence data for W19003B-1 and W19003B-4 is available from the NCBI Sequence Read Archive (SRA) under BioProject ID PRJNA934950.

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Acknowledgements

Financial support was provided by the USDA National Institute of Food Agriculture Award 2019-51181-30021.

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin-Madison, Madison, WI, 53706, USA

    Peyton L. Sorensen, Grace Christensen & Jeffrey B. Endelman

  2. USDA Agricultural Research Service, Madison, WI, 53706, USA

    Hari S. Karki

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  1. Peyton L. Sorensen
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  2. Grace Christensen
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  3. Hari S. Karki
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  4. Jeffrey B. Endelman
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Contributions

PS: Formal analysis, Investigation, Writing – original draft, Writing – review & editing. GC: Methodology, Writing – review & editing. HK: Investigation, Writing – review & editing. JBE: Conceptualization, Formal analysis, Funding acquisition, Supervision, Writing – review & editing.

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Correspondence to Jeffrey B. Endelman.

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Sorensen, P.L., Christensen, G., Karki, H.S. et al. A KASP Marker for the Potato Late Blight Resistance Gene RB/Rpi-blb1. Am. J. Potato Res. 100, 240–246 (2023). https://doi.org/10.1007/s12230-023-09914-6

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