Abstract
Potato late blight, caused by the oomycete pathogen, Phytophthora infestans de Bary, is one of the most devastating plant diseases in the world. It is of great importance to screen disease resistance germplasms and elucidate the molecular mechanisms underlying the resistance. In a previous study, Huasong 7, a tetraploid potato variety, was identified to possess resistance to multiple P. infestans races at various levels. In the present study, Huasong 7 was inoculated with an incompatible isolate 428–2 and a compatible P. infestans isolate T30–4, and gene expression was profiled at 0, 24, 48, and 72 h post inoculation. The results showed that more gene expression differentiation was observed in the compatible interaction induced by T30–4 than that in the incompatible interaction induced by 428–2. The differentially expressed genes induced by 428–2 were significantly enriched in GO terms associated with immune responses and the regulation of defense responses, as well as KEGG pathways related to antioxidant activities, for example, the glutathione metabolism. In contrast, differentially expressed genes induced by T30–4 were significantly enriched in GO terms associated with transcription, translation, and biosynthesis of macromolecules, as well as disease resistance related KEGG pathways, such as phosphoinositide metabolism, phospholipid signaling system, and receptor-mediated endocytosis. Our findings indicated that Huasong 7 responds to incompatible P. infestans isolate through disease resistance and host defense related pathways; while it responds to compatible isolate T30–4 with basic responses. It is also possible that T30–4 contains other unknown Avr genes. In summary, our study could provide an important reference for a better understanding of potato late blight resistance mechanisms of both compatible and incompatible interactions.
Resumen
El tizón tardío de la papa, causado por el patógeno oomiceto Phytophthora infestans de Bary, es una de las enfermedades de plantas más devastadoras en el mundo. Es de gran importancia probar resistencia a la enfermedad de germoplasmas y elucidar los mecanismos moleculares subyacentes a la resistencia. En estudio previo, Huasong 7, una variedad de papa tetraploide, se identificó como teniendo resistencia a múltiples razas de P. infestans a varios niveles. En el presente estudio, se inoculó a Huasong 7 con el aislamiento incompatible 428–2 y uno compatible, T30–4 de P. infestans, y la expresión de los genes se definió a los 0, 24, 48 y 72 hs después de la inoculación. Los resultados mostraron que se observó más diferenciación de expresión de genes en la interacción compatible inducida por T30–4 que aquella incompatible inducida por 428–2. Los genes expresados diferencialmente inducidos por 428–2 fueron enriquecidos significativamente en términos GO asociados con respuestas inmunes y la regulación de respuestas de defensa, así como en las rutas KEGG relacionadas a actividades antioxidantes, por ejemplo, el metabolismo del gutation. En contraste, los genes expresados diferencialmente inducidos por T30–4 se enriquecieron significativamente en términos de GO asociados con transcripción, traducción y biosíntesis de macromoléculas, así como resistencia a la enfermedad relacionada a las rutas KEGG, como el metabolismo fosfoinosítido, el sistema de señalización de fosfolípidos y la endocitosis mediada por receptores. Nuestros hallazgos indicaron que Huasong 7 responde al aislamiento de P. infestans incompatible por vía de rutas relacionadas a resistencia a la enfermedad y defensa del hospedero, mientras que responde al aislamiento compatible T30–4 con respuestas básicas. También es posible que T30–4 contenga otros genes Avr desconocidos. En resumen, nuestro estudio pudo proporcionar una referencia importante para un mejor entendimiento de los mecanismos de resistencia del tizón tardío de la papa de ambas interacciones, compatible e incompatible.
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This work was supported by grants from Major special funds for science and technology of Inner Mongolia (zdzx2018019); Animal and plant breeding project for transformation of scientific achievements in Inner Mongolia Agricultural University (YZGC2017006); Key project of science and technology plan of Inner Mongolia (201602048). The authors declare that the research was conducted in the absence of any financial and non-financial relationships that could be construed as a potential conflict of interest.
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Liu, J., Yu, Z., Yang, Y. et al. Gene Profiling of Compatible and Incompatible Interactions between Potato and Phytophthora infestans. Am. J. Potato Res. 97, 376–392 (2020). https://doi.org/10.1007/s12230-020-09789-x
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DOI: https://doi.org/10.1007/s12230-020-09789-x