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Predictive Markers for Cold-Induced Sweetening Resistance in Cold Stored Potatoes (Solanum tuberosum L.)

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Abstract

An approach has been developed to screen a large number of potato clones for cold induced sweetening (CIS) resistance in breeding programs. Two key enzymes responsible for reducing sugar accumulation during cold storage were identified. Clones with the A-II isozymes of UDP-glucose pyrophosphorylase coupled with low activity of vacuolar acid invertase enzyme had increased resistance to CIS by forming less suc, which is subsequently hydrolyzed to the undesirable reducing sugars, glc and fru. Six named cultivars and 192 genetically diverse clones from various breeding programs in USA were analyzed over two years for the two key enzymes and sugar concentration in cold stored tubers. The predictability for CIS resistance during cold storage was 94% both years. Clones classified as class A accumulated low concentration of reducing sugar glc during cold storage. It is suggested that these two predictor enzymes can be used for screening parents and selections in potato breeding program.

Resumen

Se ha desarrollado una estrategia para evaluar un gran número de clones de papa para resistencia al endulzamiento inducido por frío (CIS) en programas de mejoramiento. Se identificaron dos enzimas clave como responsables de la acumulación de azúcar reductor durante el almacenamiento en frío. Los clones con las isoenzimas A-II de UDP-glucosa pirofosforilasa, acopladas con la baja actividad de la enzima vacuolar ácido invertasa, han aumentado la resistencia a CIS mediante la formación de menos sac, que es subsecuentemente hidrolizada a los azúcares reductores indeseables glc y fru. Se analizaron seis variedades con nombre y 192 clones genéticamente diversos de varios programas de mejoramiento en los EUA, durante dos años para las dos enzimas clave y concentración de azúcar en tubérculos almacenados en frío. La predicción de la resistencia a CIS durante el almacenamiento en frío fue de 94% en ambos años. Los clones clasificados como clase A acumularon baja concentración de los azucares reductores glc durante el almacenamiento en frío. Se sugiere que estas dos enzimas de predicción pueden usarse para analizar progenitores y selecciones en programas de mejoramiento en papa.

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Abbreviations

CIS:

Cold induced sweetening

DTT:

Dithiothreitol

GFP:

Glucose forming potential

HEPES:

(N-[2-hydroxyl]piperazine-N′[ethanesulfonic acid])

PAGE:

Poly acrylamide gel electrophoresis

PGM:

Phosphoglucomutase

PMSF:

Phenylmethylsulfonyl fluoride

PVDF:

Polyvinylidene fluroide

PVPP:

Poly venylpoly-pyrrolidone

RC:

Recombinant

SPS:

Suc-6-phosphate synthase

UGPase:

UDP-Glc pyrophosphorylase

AcInv:

Acid invertase

Units:

μmol glc formed h−1 mg−1 protein

FWT:

Fresh Weight tuber.

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Acknowledgements

The funding for this research project was provided by Potatoes USA. Author thanks Dr. James Crants for statistical analysis of data. Drs. Florian Lauer and Carl Rosen for the critical review of the manuscript

Patent Application Number

14/535,634. U.S. Patent and Trademark Office: U.S. DEPARTMENT OF COMMERCE

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Authors and Affiliations

  1. Department of Soil, Water and Climate, University of Minnesota, 1991 Upper Bufford Circle, St. Paul, MN, 55108, USA

    Sanjay K. Gupta

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Correspondence to Sanjay K. Gupta.

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Gupta, S.K. Predictive Markers for Cold-Induced Sweetening Resistance in Cold Stored Potatoes (Solanum tuberosum L.). Am. J. Potato Res. 94, 297–305 (2017). https://doi.org/10.1007/s12230-017-9565-5

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