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Roots of Rosa majalis Herrm. as a source of antioxidants and anti-influenza agents

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Abstract

Rosa majalis Herrm. (Rosaceae) is a shrub widespread throughout Eurasia. In West Siberia, it is cultivated as an ornamental plant and raw material for the manufacture of fruit multivitamin concentrates. Meanwhile, metabolites and biological activities of its roots are poorly studied. The aim of this investigation was to evaluate the potential of ethyl acetate (EtOAc), butanol (ButOH), aqueous ethanol (EtOH), and aqueous (H2O) extracts of R. majalis roots as sources of antioxidant and anti-influenza compounds. Spectrophotometry and HPLC were used for metabolite quantification. Antioxidant activities were determined via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The antiviral activities were assessed as half-maximal inhibitory concentrations (IC50). The EtOAc extract manifested the highest concentrations of phenolic compounds (53.79 mg g−1 of dry extract) and saponins (48.92 mg g−1). Total gallic acid was the main phenolic compound in all extracts (24.98–38.68 mg g−1). Catechin, free ellagic acid, and total ellagic acid had the highest levels in the EtOAc, H2O, and EtOH extracts, respectively (30.83, 22.38, and 34.95 mg g−1). The EtOAc extract showed the highest free-radical scavenging activity (2.31 trolox equivalents) and anti-influenza effects (IC50 61.8 and 50.3 µg mL−1) against A/Aichi/2/68 (H3N2) and A/Chicken/05/2005 (H5N1), respectively. Our findings indicate good potential of these extracts for drug design against free-radical damage and influenza virus.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

Plant material from the collection of CSBG SB RAS was used: unique scientific unit (USU) 440534: “Collection of living plants indoors and outdoors.”

Funding

This work was supported by government-funded projects of the CSBG SB RAS (#AAAA-A21-121011290025-2) and the State assignment of State Research Centre of Virology and Biotechnology “Vector,” Federal Service for Surveillance on Consumer Rights Protection and Human Well-being, Russia (30/21).

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Authors and Affiliations

  1. Central Siberian Botanical Garden, Siberian Branch of Russian Academy of Sciences, 101 Zolotodolinskaya Str, Novosibirsk, Russian Federation, 630090

    Evgeniya A. Karpova, Vera A. Kostikova, Elena P. Khramova, Tatiana M. Shaldaeva & Olga Yu. Vasil’eva

  2. Federal Budgetary Research Institution, State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being, Koltsovo, Novosibirsk Oblast, Russian Federation, 630559

    Natalia A. Mazurkova, Ekaterina I. Filippova, Oleg Yu. Mazurkov & Elena V. Makarevich

Authors
  1. Evgeniya A. Karpova
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  2. Vera A. Kostikova
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  3. Elena P. Khramova
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  4. Tatiana M. Shaldaeva
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  5. Olga Yu. Vasil’eva
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  6. Natalia A. Mazurkova
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  9. Elena V. Makarevich
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Contributions

EAK performed studies by means of HPLC, carried out statistical analyses, and wrote the manuscript; VAK performed studies by means of HPLC, drafted the manuscript, and provided critical readings of the manuscript; EPK designed the study and provided critical readings of the manuscript; TMS carried out the DPPH assay and spectrophotometric analyses; OYV collected the samples of the plants, designed the study, and revised the manuscript; NAM designed the antiviral assay and provided critical readings of the manuscript; EIF, OYM, and EVM performed the antiviral assay, prepared tables, and wrote the manuscript. All authors discussed the results and commented on the manuscript.

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Correspondence to Evgeniya A. Karpova.

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Karpova, E.A., Kostikova, V.A., Khramova, E.P. et al. Roots of Rosa majalis Herrm. as a source of antioxidants and anti-influenza agents. Rend. Fis. Acc. Lincei 35, 97–108 (2024). https://doi.org/10.1007/s12210-024-01230-3

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