Abstract
The present study investigates the effects of heat stress on the kidney in broilers, based on previous findings which showed that heat stress caused cardiac damage in broilers. Further, the possible renoprotective role of aspirin and the heat shock proteins HSP60 and HSP47 was also investigated. The enzyme levels of urea and uric acid, which are indicators of renal damage, and lactate dehydrogenase, an indicator of oxidative damage, were measured in chickens that were only exposed to heat stress, chickens that were pretreated with aspirin before heat stress, and chickens that were only treated with aspirin. Further, histological examination of renal tissue from the three groups was also performed. Finally, expression of HSP60 and HSP47 was also examined. In the heat stress group, the enzyme measurements were indicative of renal dysfunction and oxidative damage, and the histological findings were indicative of renal ischemia and damage. Aspirin seemed to have a protective effect against the renal damage caused by the stress, based on the enzyme measurements and histopathological findings in the aspirin-treated group. The findings also indicate that aspirin may induce HSP60 and HSP47 expression in renal cells. Finally, the expression patterns of HSP60 and HSP47 indicated that they may play a renoprotective role, as their expression was higher in the aspirin-treated groups. In conclusion, the present findings show that heat stress causes renal damage in poultry and that aspirin may play a protective role against this damage via pathways that involve HSP60 and HSP47.
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30 January 2020
Due to an unfortunate mistake, an incorrect image appeared in Fig.��3 (Aspirin 24��h) of the original publication.
References
Allakhverdiev SI, Kreslavski VD, Klimov VV, Los DA, Carpentier R, Mohanty P (2008) Heat stress: an overview of molecular responses in photosynthesis. Photosynth Res 98:541–550
Bao E, Sultan KR, Nowak B, Hartung J (2008) Expression and distribution of heat shock proteins in the heart of transported pigs. Cell Stress Chaperones 13:459–466
Barrow MW, Clark KA (1998) Heat-related illnesses. Am Fam Physician 58:749–756
Blum A, Miller H (1998) Role of cytokines in heart failure. Am Heart J 135:181–186
Bounda GA, Zhou W, Wang D, Yu F (2015) Rhein elicits in vitro cytotoxicity in primary human liver HL-7702 cells by inducing apoptosis through mitochondria-mediated pathway. Evid Based Complement Altern Med 6–14 2015, 19 pages
Bruschi SA, West KA, Crabb JW, Gupta RS, Stevens JL (1993) Mitochondrial HSP60 (P1 protein) and a HSP70-like protein (mortalin) are major targets for modification during S-(1,1,2,2-tetrafluoroethyl)-L-cysteine-induced nephrotoxicity. J Biol Chem 268:23157–23161
Bukau B, Horwich AL (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92:351–366
Buriro R, Lv YJ, Ali I, Tang S, Liu ZJ, Zhang M, Adem A, Hartung J, Bao ED (2012) Temporal variations of Hsp60 and HSF-1 in primary rat myocardial cells in vitro under heat stress. Genet Mol Res GMR 12:3003–3016
Deluca HF, Engstrom GW (1961) Calcium uptake by rat kidney mitochondria. Proc Natl Acad Sci U S A 47:1744–1750
Reading DS, Hallberg RL, Myers AM (1989) Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor. Nature 337:655–659
Ebert MP et al (2005) Protective role of heat shock protein 27 in gastric mucosal injury. J Pathol 207:177–184. https://doi.org/10.1002/path.1815
Endo S et al (2007) Geranylgeranylacetone, an inducer of the 70-kDa heat shock protein (HSP70), elicits unfolded protein response and coordinates cellular fate independently of HSP70. Mol Pharmacol 72:1337–1348. https://doi.org/10.1124/mol.107.039164
Erlejman AG, Lagadari M, Toneatto J, Piwien-Pilipuk G, Galigniana MD (2014) Regulatory role of the 90-kDa-heat-shock protein (Hsp90) and associated factors on gene expression. Biochim Biophys Acta BBA-Gene Regul Mech
Evans M, Fored CM, Bellocco R, Fitzmaurice G, Fryzek JP, Mclaughlin JK, Nyrén O, Elinder CG (2009) Acetaminophen, aspirin and progression of advanced chronic kidney disease. Nephrol Dial Transpl 24:1908–1918
Ghavami A, Nutt MP, Hardy SP (2002) Heat shock protein and high-dose aspirin: effects on random skin flap survival in a rat model. Ann Plast Surg 48:60–67
Hamilton AM, Heikkila JJ (2006) Examination of the stress-induced expression of the collagen binding heat shock protein, hsp47, in Xenopus laevis cultured cells and embryos. Comp Biochem Physiol A Mol Integr Physiol 143:133–141
Holm T, Bruchmann J, Scheynius A, Langel Ü (2012) Cell-penetrating peptides as antifungals towards Malassezia sympodialis. Lett Appl Microbiol 54:39–44
Jurivich DA, Sistonen L, Kroes RA, Morimoto RI (1992) Effect of sodium salicylate on the human heat shock response. Science 255:1243–1245
Kuo T, Malik Z, Kott KS, Chen L, Knowlton AA (2012) Cardiac myocyte exosome stability and function: role of HSP60. FASEB J 26:1064–1066
Lebret T, Watson R, Molinie V, O’Neill A, Botto H, Fitzpatrick JM (2003) Heat shock proteins -HSP27, HSP60, HSP70, HSP90—expression in bladder cancer. Cancer 98:88–88
Lin H, Decuypere E, Buyse J (2004) Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus): 2. Short-term effect. Comp Biochem Physiol B Biochem Mol Biol 139:745–751
Mao WP, Ye JL, Guan ZB, Zhao JM, Zhang C, Zhang NN, Jiang P, Tian T (2007) Cadmium induces apoptosis in human embryonic kidney (HEK) 293 cells by caspase-dependent and -independent pathways acting on mitochondria. Toxicol in Vitro 21:343–354
Murray WM (1997) Inflammation, aspirin, and the risk of cardiovascular disease. N Engl J Med 337:422–424
Nagata K, Hosokawa N (1996) Regulation and function of collagen-specific molecular chaperone, HSP47. Cell Struct Funct 21:425–430
Razzaque MS, Taguchi T (1999) Localization of HSP47 in cisplatin-treated rat kidney: a possible role in tubulointerstitial damage. Clin Exp Nephrol 3:222–228
Santopadre G, Bolton S (1967) Interaction of aspirin with urea in water. J Pharm Pharmacol 19:550–551
Somji, S., Todd, J.H., Sens, M.A., Garrett, S.H., and Sens, D.A. (2000) Expression of heat shock protein 60 in human proximal tubule cells exposed to heat, sodium arsenite and CdCl(2). Toxicol Lett 115:127–136
Song E, Tang S, Xu J, Yin B, Bao E, Hartung J (2016) Lenti-siRNA Hsp60 promote bax in mitochondria and induces apoptosis during heat stress. Biochem Biophys Res Commun 481:125–131
Tang H, Chen Y, Liu X, Wang S, Lv Y, Wu D, Wang Q, Luo M, Deng H (2016) Downregulation of HSP60 disrupts mitochondrial proteostasis to promote tumorigenesis and progression in clear cell renal cell carcinoma. Oncotarget 7:38822–38834
Tang S, Lv Y, Chen H, Adam A, Cheng Y, Hartung J, Bao E (2014) Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro. PLoS One 9:e86937
Tian J, Zeng G, Pang X, Liang M, Zhou J, Fang D, Liu Y, Li D, Jiang L (2012) Identification and immunogenicity of two new HLA-A*0201-restricted CD8+ T-cell epitopes on dengue NS1 protein. Int Immunol 24:207–218
Wakefield AP, Ogborn MR, Ibrahim N, Aukema HM (2012) A dietary conjugated linoleic acid treatment that slows renal disease progression alters renal cyclooxygenase-2-derived prostanoids in the Han: SPRD-cy rat. J Nutr Biochem 23:908–914
Wali RK, Wali RK (2010) Aspirin and the prevention of cardiovascular disease in chronic kidney disease. J Am Coll Cardiol 56:966–968
Wischmeyer PE (2002) Glutamine and heat shock protein expression. Nutrition 18:225–228
Wu D, Xv J, Song E, Tang S, Zhang X, Kemper N, Hartung J, Bao E (2015) Acetyl salicylic acid protected against heat stress damage in chicken myocardial cells and may associate with induced Hsp27 expression. Cell Stress Chaperones 20:687–696
Wu D, Zhang M, Xu J, Song E, Lv Y, Tang S, Zhang X, Hartung J, Endong B (2016) In vitro evaluation of aspirin-induced HspB1 against heat stress damage in chicken myocardial cells. Cell Stress Chaperones 21:405–413
Xiao HB, Liu RH, Ling GH, Xiao L, Xia YC, Liu FY, Li J, Liu YH, Chen QK, Lv JL (2012) HSP47 regulates ECM accumulation in renal proximal tubular cells induced by TGF-β1 through ERK1/2 and JNK MAPK pathways. Am J Physiol Renal Physiol 303:F757–F765
Yan J, Bao E, Yu J (2009) Heat shock protein 60 expression in heart, liver and kidney of broilers exposed to high temperature. Res Vet Sci 86:533–538
Zhang X, Qian Z, Zhu H, Tang S, Wu D, Zhang M, Kemper N, Hartung J, Bao E (2016) HSP90 gene expression induced by aspirin is associated with damage remission in a chicken myocardial cell culture exposed to heat stress. Br Poult Sci 57:462–473
Funding
This work was supported by the Fundamental Research Funds for the Central Universities [grant number KJQN201709], the National Natural Science Foundation of China [grant number 31672027], the Jiangsu Natural Science Foundation of China [grant number BK20160732], and the China Postdoctoral Science Foundation [grant number 2016M591860].
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Tang, S., Zhou, S., Yin, B. et al. Heat stress-induced renal damage in poultry and the protective effects of HSP60 and HSP47. Cell Stress and Chaperones 23, 1033–1040 (2018). https://doi.org/10.1007/s12192-018-0912-3
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DOI: https://doi.org/10.1007/s12192-018-0912-3