Abstract
The development of rapid and effective detection of pathogens is necessary to prevent bacterial infection. Herein, we constructed a novel polymerase chain reaction-dynamic light scattering (PCR-DLS) sensing assay for detecting Salmonella sensitively. First, the invA gene specific for Salmonella was designed with upstream and downstream primers containing oxyethylene glycol bridge blockers which could hybridize with DNA-functionalized gold nanoparticle probes (probes-AuNPs). And the probes were hybridized complementary to the trailing end of the primers, which resulted in the aggregation of AuNPs and the change of particle diameter that could be measured by DLS. The results show that the PCR-DLS assay could improve the detection sensitivity and the limit of detection (LOD) was as low as 4.0 CFU/mL in pure culture and 1.8 × 102 CFU/mL in the spiked skim milk samples, which the detection time was less than 2 h. In conclusion, this work provides a promising strategy for developing the DLS technique of detecting foodborne pathogens.
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The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Funding
The work was supported by the Research Foundation from Academic and Technical Leaders of Major Disciplines in Jiangxi Province, China (20194BCJ22004).
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Qian Xu: Data curation and Writing original draft. Guoyang Xie: Investigation and Methodology. Ju Liu: Formal analysis. Yu Ye: Resources. Baoqing Zhou: Resources. Ping Tong: Software. Zoraida P. Aguilar: Writing—review & editing. Hengyi Xu: Methodology, Funding acquisition, Supervision, and Writing—review & editing.
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Hengyi Xu declares that he has no conflict of interest. Qian Xu declares that she has no conflict of interest. Guoyang Xie declares that he has no conflict of interest. Ju Liu declares that he has no conflict of interest. Yu Ye declares that she has no conflict of interest. Baoqing Zhou declares that he has no conflict of interest. Ping Tong declares that she has no conflict of interest. Zoraida P. Aguilar declares that she has no conflict of interest.
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Xu, Q., Xie, G., Liu, J. et al. An Ultrasensitive Method Using Polymerase Chain Reaction-Dynamic Light Scattering for Detection of Salmonella in Milk. Food Anal. Methods 16, 1337–1346 (2023). https://doi.org/10.1007/s12161-023-02508-2
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DOI: https://doi.org/10.1007/s12161-023-02508-2