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Monitoring Seasonal Differences on Contamination and Carry-over of Zearalenone from Feedstuffs to Foods by Molecularly Imprinted Solid-phase Extraction and HPLC-FLD

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Abstract

Contamination and carry-over of trace zearalenone (ZON) and its metabolite α-zearalanol (α-ZOL) from different feedstuffs to foods in different seasons were monitored by molecularly imprinted solid-phase extraction (MISPE) and high-performance liquid chromatography with fluorescence detector (HPLC-FLD) for the first time. Molecularly imprinted polymers (MIPs) bond ZON and α-ZOL selectively were prepared using cyclododecanyl-2,4-dihydroxybenzoate (CDHB) as the pseudo template. By using MIPs as sorbents of MISPE, selective enrichment of ZON and α-ZOL by MISPE increased the accuracy of HPLC-FLD. Seasonal differences on ZON contamination were observed in different feedstuffs and raw milk. But only the carry-over of ZON from cow feedstuffs to raw milk was observed. No α-ZOL contamination was detected. The humid and warm weather in April and July in Wuhan increased ZON contamination levels and the risk of ZON transmission from cow feedstuffs to milk. Seasonal differences on ZON contamination and carry-over indicate the necessity of seasonal-dependent mycotoxin monitoring and safety evaluation.

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Funding

This study was funded by the National Natural Science Foundation of China (Grant No. 21277054), the Scientific Research Project of Wuhan City (Grant No. 2016060101010068), the Natural Science Foundation of Hubei Province (Grant No. 2016CFB177), the Natural Science Foundation of Shandong Province (Grant No. ZR2016HP15), and the Health & Family Planning Scientific Research Project of Wuhan City (Grant No. WG16C03 and WG17C03).

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Author notes

  1. Ya-ting Wu and Chang-gang Huang contributed equally to this work.

Authors and Affiliations

  1. Institute of Environmental Health and Food Safety, Wuhan Center for Disease Control and Prevention, Wuhan, 430024, Hubei, China

    Ya-ting Wu & Chang-gang Huang

  2. Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, 430030, Hubei, China

    Ning Li, Jun-feng Qi & Bin Lu

  3. School of Public Health and Management, Binzhou Medical University, #346 Guanhai Road, Yantai, 264003, Shandong, China

    Ning Li

  4. Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science & Technology, #100 Hongkong Road, Wuhan, 430015, Hubei, China

    Yi-Yang Guo

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  1. Ya-ting Wu
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  2. Chang-gang Huang
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Correspondence to Yi-Yang Guo or Bin Lu.

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Ya-ting Wu declares that he has no conflict of interest. Chang-gang Huang declares that he has no conflict of interest. Ning Li declares that he has no conflict of interest. Jun-feng Qi declares that he has no conflict of interest. Yi-Yang Guo declares that he has no conflict of interest. Bin Lu declares that he has no conflict of interest.

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Wu, Yt., Huang, Cg., Li, N. et al. Monitoring Seasonal Differences on Contamination and Carry-over of Zearalenone from Feedstuffs to Foods by Molecularly Imprinted Solid-phase Extraction and HPLC-FLD. Food Anal. Methods 15, 448–457 (2022). https://doi.org/10.1007/s12161-021-02133-x

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  • DOI: https://doi.org/10.1007/s12161-021-02133-x

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