Abstract
Validated HPLC and UV analysis were applied for quantitative determination of six isoflavones (daidzin, glycitin, genistin, daidzein, glycitein, and genistein) in soy products. In HPLC analysis, six isoflavones showed satisfactory regression (r2 > 0.99) within test ranges, and the recovery was in the range of 91.00 to 117.00%. LOD and LOQ ranged from 0.004–0.016 (µg ml−1) and 0.015–0.035 (µg ml−1), respectively. The quantified isoflavones present in prepared and commercial extracts were then subjected to principal component analysis (PCA), in conjunction with hierarchical cluster analysis (HCA), which showed differences in the isoflavone from soybeans. The employment of a simple and low-cost UV spectrophotometric methodology was also applied in quantification of same compounds in the same soy extracts. Paired t-test confirmed that there is no significant difference between the results obtained for the two methods. Our results revealed that the developed methods have potential perspective for the original discrimination and quality control of isoflavones in soybean-based products employing a rapid, fast, and not-expensive method.
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References
Ahad T, Nissar J (2017) Fingerprinting in determining the adultration of food. J Pharmacogn Phytochem 6:1543–1553. https://doi.org/10.13140/RG.2.2.25240.24322
Azevedo RSA, Teixeira BS, da Sauthier MC, S, et al (2019) Multivariate analysis of the composition of bioactive in tea of the species Camellia sinensis. Food Chem 273:39–44. https://doi.org/10.1016/j.foodchem.2018.04.030
Chen Y, Bin ZhuS, Xie MY et al (2008) Quality control and original discrimination of Ganoderma lucidum based on high-performance liquid chromatographic fingerprints and combined chemometrics methods. Anal Chim Acta 623:146–156. https://doi.org/10.1016/j.aca.2008.06.018
Cheynier V (2012) Phenolic compounds: from plants to foods. Phytochem Rev 11:153–177. https://doi.org/10.1007/s11101-012-9242-8
Collison MW, Delmonte P, Devore R et al (2008) Determination of total soy isoflavones in dietary supplements, supplement ingredients, and soy foods by high-performance liquid chromatography with ultraviolet detection: Collaborative study. J AOAC Int 91:489–500. https://doi.org/10.1093/jaoac/91.3.489
Embrapa Soja (2019) https://www.embrapa.br/soja/cultivos/soja1/dados-economicos. Accessed 5 Apr 2020
Erdman JW, Badger TM, Lampe JW et al (2004) Not all soy products are created equal: caution needed in interpretation of research results. J Nutr 134:1229–1233. https://doi.org/10.1093/jn/134.5.1229S
Ferreira DS, Poppi RJ, Lima Pallone JA (2015) Evaluation of dietary fiber of Brazilian soybean (Glycine max) using near-infrared spectroscopy and chemometrics. J Cereal Sci 64:43–47. https://doi.org/10.1016/j.jcs.2015.04.004
Gobbo-Neto L, Lopes NP (2007) Plantas medicinais: Fatores de influência no conteúdo de metabólitos secundários. Quim Nova 30:374–381. https://doi.org/10.1590/S0100-40422007000200026
Gomes AF, Ganzera M, Schwaiger S et al (2018) Simultaneous determination of iridoids, phenylpropanoids and flavonoids in Lippia alba extracts by micellar electrokinetic capillary chromatography. Microchem J 138:494–500. https://doi.org/10.1016/j.microc.2018.02.003
Gomes AF, Almeida MP, Leite MF et al (2019) Seasonal variation in the chemical composition of two chemotypes of Lippia alba. Food Chem 273:186–193. https://doi.org/10.1016/j.foodchem.2017.11.089
Guimarães AF, Vinhas ACA, Gomes AF et al (2020) Essential oil of Curcuma longa L. Rhizomes chemical composition, yield variation and stability. Quim Nova 43:909–913. https://doi.org/10.21577/0100-4042.20170547
ICH (2005) Validation of Analytical Procedures: Text and Methodology Q2 (R1)
IUPAC (2002) Harmonized guidelines for single-laboratory validation of methods of analysis (IUPAC Technical Report). In: Pure and Applied Chemistry. pp 835–855
Li J, He X, Li M et al (2015) Chemical fingerprint and quantitative analysis for quality control of polyphenols extracted from pomegranate peel by HPLC. Food Chem 176:7–11. https://doi.org/10.1016/j.foodchem.2014.12.040
Messina M, Messina V (2000) Soyfoods, soybean isoflavones, and bone health: a brief overview. J Ren Nutr 10:63–68. https://doi.org/10.1053/m.2000.5548
Migues VH, David JM, David JP (2020) Determination of polyphenols in Schinus terebinthifolius Raddi bark extracts and chemometric analysis. Anal Methods 12:1478–1485. https://doi.org/10.1039/d0ay00197j
Mota MD, Meira M, David JP, Ribeiro EMDO, Brandão HN, do Vale A, David JM (2021) New method for determination of trans-resveratrol for quality evaluation of red wines by multivariate calibration associated with UV-VIS Spectroscopy. Curr Anal Chem 17:1037–1043. https://doi.org/10.2174/1573411017666201229142412
Peñalvo JL, Nurmi T, Adlercreutz H (2004) A simplified HPLC method for total isoflavones in soy products. Food Chem 87:297–305. https://doi.org/10.1016/j.foodchem.2003.12.028
Shen D, Wu Q, Sciarappa WJ, Simon JE (2012) Chromatographic fingerprints and quantitative analysis of isoflavones in Tofu-type soybeans. Food Chem 130:1003–1009. https://doi.org/10.1016/j.foodchem.2011.07.121
Shim YS, Yoon WJ, Hwang JB et al (2015) Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection. Food Chem 187:391–397. https://doi.org/10.1016/j.foodchem.2015.04.077
Wang H, Murphy PA (1994) Isoflavone composition of American and Japanese Soybeans in Iowa: effects of variety, crop year, and location. J Agric Food Chem 42:1674–1677. https://doi.org/10.1021/jf00044a017
Wang C, Sherrard M, Pagadala S et al (2000) Isoflavone content among maturity group 0 to II soybeans. J Am Oil Chem Soc 77:483–487. https://doi.org/10.1007/s11746-000-0077-6
Wang Q, Ge X, Tian X et al (2013) Soy isoflavone: the multipurpose phytochemical (Review). Biomed Rep 1:697–701. https://doi.org/10.3892/br.2013.129
Xiao M, Ye J, Tang X, Huang Y (2011) Determination of soybean isoflavones in soybean meal and fermented soybean meal by micellar electrokinetic capillary chromatography (MECC). Food Chem 126:1488–1492. https://doi.org/10.1016/j.foodchem.2010.11.168
Xu CJ, Liang YZ, Chau FT, Vander HY (2006) Pretreatments of chromatographic fingerprints for quality control of herbal medicines. J Chromatogr A 1134:253–259. https://doi.org/10.1016/j.chroma.2006.08.060
Zafra-Gómez A, Garballo A, García-Ayuso LE, Morales JC (2010) Improved sample treatment and chromatographic method for the determination of isoflavones in supplemented foods. Food Chem 123:872–877. https://doi.org/10.1016/j.foodchem.2010.05.009
Zaheer K, Akhtar MH (2017) An updated review of dietary isoflavones: nutrition, processing, bioavailability and impacts on human health. Crit Rev Food Sci Nutr 57:1280–1293. https://doi.org/10.1080/10408398.2014.989958
Acknowledgements
The authors thank to Fundação de Amparo à Pesquisa da Bahia (FAPESB) for the grants and scholarship.
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CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico (# 406427/2018–6).
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Vitor Hugo Migues declares that he/she has no conflict of interest. Jorge Mauricio David declares that he/she has no conflict of interest. Angélica Ferraz Gomes declares that he/she has no conflict of interest. Juceni Pereira David declares that he/she has no conflict of interest.
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Migues, V.H., David, J.M., Gomes, A.F. et al. Determination of Soybean Isoflavone by HPLC/DAD and Simple UV Spectroscopic Analysis: a Comparative Study. Food Anal. Methods 15, 367–376 (2022). https://doi.org/10.1007/s12161-021-02120-2
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DOI: https://doi.org/10.1007/s12161-021-02120-2