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Ringer Tablet-Based Micelle-Mediated Extraction-Solvent Back Extraction Coupled with High-Performance Liquid Chromatography for Preconcentration and Determination of Neonicotinoid Pesticides

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Abstract

Ringer tablet-based micelle-mediated extraction-solvent back extraction method coupled to HPLC has been investigated for the determination of neonicotinoid pesticides in honey, cucumber, and orange samples. The method employs the entrapment of the analytes in the micelles of non-ionic surfactant (Triton X-114). By ultrasonication, the ringer tablet is dissolved, and the extractant is released into the aqueous phase as very tiny droplets to provide a cloudy solution. After centrifuging the extracted analytes into the surfactant, they are collected at the bottom of a conical test tube. Then, the analytes were back-extracted from the final micellar extract into appropriate organic solvent (1-octanol), a procedure that facilitates the analysis method. The parameters affecting the extraction efficiency were studied such as the type and amount of ringer tablet, the type and concentration of surfactant, the type and volume of the organic solvent, and the extraction time. The method showed good linearity with the correlation coefficients (R2) > 0.9, good reproducibility with the relative standard deviation (RSD) < 3% for the retention time, and < 10% for peak area, respectively. Furthermore, the low limits of detection (LOD) and the limits of quantitation (LOQ) are within the range of 0.0003–0.001 and 0.0009–0.003 µg mL−1, respectively. The result demonstrated that the present method was a good extraction efficiency and can be used for the simultaneous determination of neonicotinoid pesticides in honey, cucumber, and orange samples.

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References

  • Biever RC, Hoberg JR, Jacobson B, Dionne E, Sulaiman M, McCahon P (2003) ICON rice seed treatment toxicity to crayfish (Procambarus clarkii) in experimental rice paddies. Environ Toxicol Chem 22:167–174

    Article  CAS  Google Scholar 

  • Campillo N, Viñas P, Férez-Melgarejo G, Hernández-Córdoba M (2013) Development of an enzyme-linked immunosorbent assay for the insecticide thiamethoxam. J Agric Food Chem 61:4799–4805

    Article  CAS  Google Scholar 

  • Cazorla-Reyes R, Fernandez-Moreno JL, Romero-Gonzalez R, Frenich AG, Vidal JL (2011) Single solid phase extraction method for the simultaneous analysis of polar and non-polar pesticides in urine samples by gas chromatography and ultra high pressure liquid chromatography coupled to tandem mass spectrometry. Talanta 85:183–196

    Article  CAS  Google Scholar 

  • Chen M, Tao L, Collins EM, Austin C, Lu CS (2012) Simultaneous determination of multiple phthalate metabolites and bisphenol-A in human urine by liquid chromatography-tandem mass spectrometry. J Chromatogr B 904:73–80

    Article  CAS  Google Scholar 

  • Chitescu CL, Oosterink E, de Jong J, Maria (Linda) Stolker AA, (2012) Ultrasonic or accelerated solvent extraction followed by U-HPLC-high mass accuracy MS for screening of pharmaceuticals and fungicides in soil and plant samples. Talanta 88:653–662

    Article  CAS  Google Scholar 

  • Di Muccio A, Fidente P, Barbini DA, Dommarco R, Seccia S, Morrica P (2006) Application of solid-phase extraction and liquid chromatography–mass spectrometry to the determination of neonicotinoid pesticide residues in fruit and vegetables. J Chromatogr A 1108:1–6

    Article  Google Scholar 

  • EU pesticides database; https://ec.europa.eu/food/plant/pesticides/

  • García MDG, Galera MM, Valverde RS, Galanti A, Girotti S (2007) Column switching liquid chromatography and post-column photochemically fluorescence detection to determine imidacloprid and 6-chloronicotinic acid in honeybees. J Chromatogr A 1147:17–23

    Article  Google Scholar 

  • Jansson C, Pihlstrom T, Öterdahl BG, Markides KE (2004) A new multi-residue method for analysis of pesticide residues in fruit and vegetables using liquid chromatography with tandem mass spectrometric detection. J Chromatogr A 1023:93–104

    Article  CAS  Google Scholar 

  • Jovanov P, Guzsvány V, Franko M, Lazić S, Sakač M, Šarić B, Banjac V (2013) Multi-residue method for determination of selected neonicotinoid insecticides in honey using optimized dispersive liquid–liquid microextraction combined with liquid chromatography-tandem mass spectrometry. Talanta 111:125–133

    Article  CAS  Google Scholar 

  • Jovanov P, Guzsvány V, Lazić S, Franko M, Sakač M, Šarić L, Kos J (2015) Development of HPLC-DAD method for determination of neonicotinoids in honey. J Food Compos Anal 40:106–113

    Article  CAS  Google Scholar 

  • Kardani F, Daneshfar A, Sahrai R (2011) Determination of β-sitosterol and cholesterol in oils after reverse micelles with Triton X-100 coupled with ultrasound-assisted back-extraction by a water/chloroform binary system prior to gas chromatography with flame ionization detection. Anal Chim Acta 701:232–237

    Article  CAS  Google Scholar 

  • Liu S, Zheng Z, Wei F, Ren Y, Gui W, Wu H, Zhu G (2010) Simultaneous determination of seven neonicotinoid pesticide residues in food by ultraperformance liquid chromatography tandem mass spectrometry. J Agric Food Chem 58:3271–3278

    Article  CAS  Google Scholar 

  • Liu L, Feng T, Wang C, Wu Q, Wang Z (2014) Enrichment of neonicotinoid insecticides from lemon juice sample with magnetic three-dimensional graphene as the adsorbent followed by determination with high-performance liquid chromatography. J Sep Sci 37:1276–1282

    Article  CAS  Google Scholar 

  • Ma L, Wang Y, Li H, Peng F, Qiu B, Yang Z (2020) Development of QuEChERS-DLLME method for determination of neonicotinoid pesticide residues in grains by liquid chromatography-tandem mass spectrometry. Food Chem 331:127190

    Article  CAS  Google Scholar 

  • Ramezani Z, Mirzajani R, Kardani F (2015) A novel ultrasound-assisted back extraction reverse micelles method coupled with gas chromatography–flame ionization detection for determination of aldehydes in heated edibles oils. Food Chem 188:30–36

    Article  CAS  Google Scholar 

  • Rancan M, Rossi S, Sabatini AG (2006) Determination of thiamethoxam residues in honeybees by high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor. J Chromatogr A 1123(1):60–65

    Article  CAS  Google Scholar 

  • Rodríguez-Cabo T, Casado J, Rodríguez I, Ramil M, Cela R (2016) Selective extraction and determination of neonicotinoid insecticides in wine by liquid chromatography–tandem mass spectrometry. J Chromatogr A 1460:9–15

    Article  Google Scholar 

  • Santalad A, Srijaranai S, Burakham R, Glennon JD, Deming RL (2009) Cloud-point extraction and reversed-phase high-performance liquid chromatography for the determination of carbamate insecticide residues in fruits. Anal Bioanal Chem 394:1307–1317

    Article  CAS  Google Scholar 

  • Sanz GP, Halko R, Ferrera ZS, Rodriguez JJS (2004) Micellar extraction of organophosphorus pesticides and their determination by liquid chromatography. Anal Chim Acta 524:265–270

    Article  Google Scholar 

  • Seccia S, Fidente P, Montesano D, Morrica P (2008) Analysis of nine drugs and their cytochrome P450-specific probe metabolites from urine by liquid chromatography–tandem mass spectrometry utilizing sub 2μm particle size column. J Chromatogr A 1214:115–120

    Article  CAS  Google Scholar 

  • Seebunrueng K, Santaladchaiyakit Y, Soisungnoen P, Srijaranai S (2011) Catanionic surfactant ambient cloud point extraction and high-performance liquid chromatography for simultaneous analysis of organophosphorus pesticide residues in water and fruit juice samples. Anal Bioanal Chem 401:1703–1712

    Article  Google Scholar 

  • Shen JC, Shao XG (2006) Determination of tobacco alkaloids by gas chromatography–mass spectrometry using cloud point extraction as a preconcentration step. Anal Chim Acta 561:83–87

    Article  CAS  Google Scholar 

  • Štajnbaher D, Zupančič-Kralj L (2003) Multiresidue method for determination of 90 pesticides in fresh fruits and vegetables using solid-phase extraction and gas chromatography-mass spectrometry. J Chromatogr A 1015:185–198

    Article  Google Scholar 

  • Su Y-S, Jen J-F (2010) Determination of organophosphorous pesticides in water using in-syringe ultrasound-assisted emulsification and gas chromatography with electron-capture detection. J Chromatogr A 1217:5043–5049

  • Taliansky-Chamudis A, Gómez-Ramírez P, León-Ortega M, García-Fernández AJ (2017) Validation of a QuECheRS method for analysis of neonicotinoids in small volumes of blood and assessment of exposure in Eurasian eagle owl (Bubo bubo) nestlings. Sci Total Environ 595:93–100

    Article  CAS  Google Scholar 

  • Tanner G, Czerwenka C (2011) LC-MS/MS analysis of neonicotinoid insecticides in honey: methodology and residue findings in Austrian honeys. J Agric Food Chem 59:12271–12277

    Article  CAS  Google Scholar 

  • Wang P, Yang X, Wang J, Cui J, Dong AJ, Zhao HT, Zhang LW, Wang ZY, Xu RB, Li WJ, Zhang YC, Zhang H, Jing J (2012) Multi-residue method for determination of seven neonicotinoid insecticides in grains using dispersive solid-phase extraction and dispersive liquid–liquid micro-extraction by high performance liquid chromatography. Food Chem 134:1691–1698

    Article  CAS  Google Scholar 

  • Xiao Z, Li X, Wang X, Shen J, Ding S (2011) Determination of neonicotinoid insecticides residues in bovine tissues by pressurized solvent extraction and liquid chromatography–tandem mass spectrometry. J Chromatogr B 879:117–122

    Article  CAS  Google Scholar 

  • Yang X, Yu R, Zhang S, Cao B, Liu Z, Lei L, Li N, Wang Z, Zhang L, Zhang H, Chen Y (2014) Aqueous two-phase extraction for determination of triazine herbicides in milk by high-performance liquid chromatography. J Chromatogr B 972:111–116

    Article  CAS  Google Scholar 

  • Zhou Q, Ding Y, Xiao J (2006) Sensitive determination of thiamethoxam, imidacloprid and acetamiprid in environmental water samples with solid-phase extraction packed with multiwalled carbon nanotubes prior to high-performance liquid chromatography. Anal Bioanal Chem 385:1520–1525

    Article  CAS  Google Scholar 

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Acknowledgements

This research project was financially supported by Mahasarakham University (Fast Track 2020). We also thank Miss Kanokporn U-ra and Miss Nalita Casombute for their assistance in the laboratory.

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Authors and Affiliations

  1. Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha sarakham , 44150, Thailand

    Jitlada Vichapong & Rawikan Kachangoon

  2. Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Rodjana Burakham & Supalax Srijaranai

  3. Department of Chemistry, Faculty of Engineering, Khon Kaen Campus, Rajamangala University of Technology Isan, Khon Kaen, 40000, Thailand

    Yanawath Santaladchaiyakit

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  1. Jitlada Vichapong
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  2. Rawikan Kachangoon
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  3. Rodjana Burakham
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  4. Yanawath Santaladchaiyakit
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  5. Supalax Srijaranai
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Correspondence to Jitlada Vichapong.

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Jitlada Vichapong declares that she has no conflict of interest. Rawikan Kachangoon declares that she has no conflict of interest. Rodjana Burakham declares that she has no conflict of interest. Yanawath Santaladchaiyakit declares that he has no conflict of interest. Supalax Srijaranai declares that she has no conflict of interest.

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Vichapong, J., Kachangoon, R., Burakham, R. et al. Ringer Tablet-Based Micelle-Mediated Extraction-Solvent Back Extraction Coupled with High-Performance Liquid Chromatography for Preconcentration and Determination of Neonicotinoid Pesticides. Food Anal. Methods 15, 970–980 (2022). https://doi.org/10.1007/s12161-021-02067-4

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