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A Novel Paper-Based Capacitance Mast Cell Sensor for Evaluating Peanut Allergen Protein Ara h 2

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Abstract

A novel paper-based capacitance mast cell sensor has been designed and developed for real-time monitoring of the major peanut allergen Ara h 2. In this study, we created the 3D paper chip printed with carbon electrodes as a non-contact capacitance sensing platform. To improve the conductivity and biocompatibility of the cellulose paper, a polyvinyl alcohol (PVA)-gelatin methacryloyl (GelMA)-nano-hydroxyapatite (nHAP) composite hydrogel (PGHAP gel) was employed to fabricate the prepared paper chip. When rat basophilic leukemia mast cells (RBL-2H3) are immobilized and cultured in the 3D culture system consisting of PGHAP gel and paper fibers, identification signals of Ara h 2 could be specifically monitored non-contact and real-time by capacitance change measurement. This 3D structure combined with time-lapse monitoring completes the capacitance cell sensor. Results indicate that Ara h 2 has given a remarkable decrease to the capacitance in dose-dependent range from 0.1 to 100 ng/mL. Therefore, the real-time cell allergic response could be accurately monitored by this low-cost, disposable cell sensor, which supplies a novel and effective pathway for the rapid and accurate evaluation for food allergens.

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References

  • Almuhsen S, Clarke AE, Kagan RS (2008) Peanut allergy: an overview. Allergy Asthma Cl Im,4,4(2008-12-15) 4(4):139

    Article  Google Scholar 

  • Anzengruber J, Bublin M, Bönisch E, Janesch B, Tscheppe A, Braun ML, Varga EM, Hafner C, Breiteneder H, Schäffer C (2017) Lactobacillus buchneri S-layer as carrier for an Ara h 2-derived peptide for peanut allergen-specific immunotherapy. Mol Immunol 85:81–88

    Article  CAS  Google Scholar 

  • Arya SK, Zhurauski P, Jolly P, Batistuti MR, Mulato M, Estrela P (2018) Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum. Biosens Bioelectron 102:106–112

    Article  CAS  Google Scholar 

  • Cai Y, You J, You Z, Dong F, Du S, Zhang L (2018) Profuse color-evolution-based fluorescent test paper sensor for rapid and visual monitoring of endogenous Cu2+ in human urine. Biosens Bioelectron 99:332–337

    Article  CAS  Google Scholar 

  • Campuzano S, Montiel RV, Reviejo ÁJ, Pingarrón JM (2016) Electrochemical biosensors for food security: allergens and adulterants detection. Biosens Secur Bioterrorism Appl:287–307

  • Chang AS, Sreedharan A, Schneider KR (2013) Peanut and peanut products: a food safety perspective. Food Control 32(1):296–303

    Article  CAS  Google Scholar 

  • Gladman AS, Matsumoto EA, Nuzzo RG, Mahadevan L, Lewis JA (2016) Biomimetic 4D printing. Nat Mater 15(4):413–418

    Article  CAS  Google Scholar 

  • Graham AC, Temple RM, Obar JJ (2015) Mast cells and influenza a virus: association with allergic responses and beyond. Front Immunol 6:238

    PubMed  PubMed Central  Google Scholar 

  • Hossain SZ, Brennan JD (2011) β-Galactosidase-based colorimetric paper sensor for determination of heavy metals. Anal Chem 83(22):8772–8778

    Article  CAS  Google Scholar 

  • Jiang D, Jiang H, Ji J, Sun X, Qian H, Zhang G, Tang L (2014) Mast-cell-based fluorescence biosensor for rapid detection of major fish allergen parvalbumin. J Agric Food Chem 62(27):6473–6480

    Article  CAS  Google Scholar 

  • Kim MN, Lee KE, Hong JY, Kim KW, Kim KE, Sohn MH, Park JW (2016) IgE cross-reactivity of peanut with walnut and soybean in children with food allergy. Allergol Immunopathol 44(6):524–530

    Article  CAS  Google Scholar 

  • Klotz BJ, Gawlitta D, Rosenberg AJ, Malda J, Melchels FP (2016) Gelatin-methacryloyl hydrogels: towards biofabrication-based tissue repair. Trends Biotechnol 34(5):394–407

    Article  CAS  Google Scholar 

  • Kukkonen AK, Pelkonen AS, Mäkinen-Kiljunen S, Voutilainen H, Mäkelä MJ (2015) Ara h 2 and Ara 6 are the best predictors of severe peanut allergy: a double-blind placebo-controlled study. Allergy 70(10):1239–1245

    Article  CAS  Google Scholar 

  • Liu C-C, Wang Y-N, Fu L-M, Chen K-L (2018) Microfluidic paper-based chip platform for benzoic acid detection in food. Food Chem 249:162–167

    Article  CAS  Google Scholar 

  • Maleki SJ, Kopper RA, Shin DS, Park CW, Compadre CM, Sampson H, Burks AW, Bannon GA (2000) Structure of the major peanut allergen Ara h 1 may protect IgE-binding epitopes from degradation. J Immunol 164(11):5844–5849

    Article  CAS  Google Scholar 

  • Montiel RV, Pellicanò A, Campuzano S, Reviejo ÁJ, Cosio MS, Pingarrón JM (2016) Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2. Sensors Actuators B Chem 236:825–833

    Article  CAS  Google Scholar 

  • Niyomdecha S, Limbut W, Numnuam A, Kanatharana P, Charlermroj R, Karoonuthaisiri N, Thavarungkul P (2018) Phage-based capacitive biosensor for Salmonella detection. Talanta 188:658–664

    Article  CAS  Google Scholar 

  • O'B HJ (2015) The threshold concept in food safety and its applicability to food allergy. Allergy 56(s67):86–90

    Google Scholar 

  • Peng J, Song S, Liu L, Kuang H, Xu C (2015) Development of sandwich ELISA and immunochromatographic strip for the detection of peanut allergen Ara h 2. Food Anal Methods 8(10):2605–2611

    Article  Google Scholar 

  • Renz H, Allen KJ, Sicherer SH, Sampson HA, Lack G, Beyer K, Oettgen HC (2018) Food allergy. Nat Rev Dis Primers 4:17098

    Article  Google Scholar 

  • Rong C, Jing XU, Yi-Chen LI, Jun-Yi XU, Zheng QY, Cao JJ (2012) The advances in peanut allergen detection technology. J Food Saf Qual 3(4):254–258

    CAS  Google Scholar 

  • Sobhan A, Oh JH, Park MK, Kim SW, Park C, Lee J (2017) Single walled carbon nanotube based biosensor for detection of peanut allergy-inducing protein ara h1. Korean J Chem Eng 23:1–7

    Google Scholar 

  • Stephan O, Vieths S (2004) Development of a real-time PCR and a sandwich ELISA for detection of potentially allergenic trace amounts of peanut (Arachis hypogaea) in processed foods. J Agric Food Chem 52(12):3754–3760

    Article  CAS  Google Scholar 

  • Sun X, Ji J, Jiang D, Li X, Zhang Y, Li Z, Wu Y (2013) Development of a novel electrochemical sensor using pheochromocytoma cells and its assessment of acrylamide cytotoxicity. Biosens Bioelectron 44:122–126

    Article  CAS  Google Scholar 

  • Teengam P, Siangproh W, Tuantranont A, Henry CS, Vilaivan T, Chailapakul O (2017) Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus. Anal Chim Acta 952:32–40

    Article  CAS  Google Scholar 

  • Vasilescu A, Nunes G, Hayat A, Latif U, Marty JL (2016) Electrochemical affinity biosensors based on disposable screen-printed electrodes for detection of food allergens. Sensors 16(11):1863

    Article  CAS  Google Scholar 

  • Wang L, Chen W, Xu D, Shim BS, Zhu Y, Sun F, Liu L, Peng C, Jin Z, Xu C (2009) Simple, rapid, sensitive, and versatile SWNT− paper sensor for environmental toxin detection competitive with ELISA. Nano Lett 9(12):4147–4152

    Article  CAS  Google Scholar 

  • Yu C, Wang Q, Li W, Li Y, Liu S, Bao N, Gu H (2015) Based cell impedance sensor and its application for cytotoxic evaluation. Nanotechnology 26(32):325501

    Article  CAS  Google Scholar 

  • Zeinabad HA, Ghourchian H, Falahati M, Fathipour M, Azizi M, Boutorabi SM (2018) Ultrasensitive interdigitated capacitance immunosensor using gold nanoparticles. Nanotechnology 29(26):265102

    Article  CAS  Google Scholar 

  • Zhu C, Dan D, Lin Y (2016) Graphene-like 2D nanomaterial-based biointerfaces for biosensing applications. Biosens Bioelectron 89(Pt 1):43–55

    PubMed  Google Scholar 

  • Zhu Y, Lu W, Guo Y, Chen Y, Wu Y, Lu H (2018) Biocompatible, stretchable and mineral PVA–gelatin–nHAP hydrogel for highly sensitive pressure sensors. RSC Adv 8(65):36999–37007

    Article  CAS  Google Scholar 

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Funding

This work was supported by the National Natural Science Foundation of China (No. 31601535), the Natural Science Foundation of Jiangsu Province (No. BK20160459, BK20180160), the Science and Technology Development Project of Nanjing (201716006), the Special Program of the State Administration for Market Regulation (2019YJ047), the Science and Technology Program of Nanjing Administration for Market Regulation (Kj2019042), and the National Key Research and Development Program of China (2016YFD0400206-3, 2016YFD0400201),

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Authors and Affiliations

  1. College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, Jiangsu, 210023, People’s Republic of China

    Donglei Jiang & Lifeng Wang

  2. Nanjing Institute for Food and Drug Control, Nanjing, Jiangsu, 211198, People’s Republic of China

    Hui Jiang

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  1. Donglei Jiang
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  2. Hui Jiang
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  3. Lifeng Wang
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Correspondence to Lifeng Wang.

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Donglei Jiang declares that he has no conflict of interest. Hui Jiang declares that she has no conflict of interest. Lifeng Wang declares that he has no conflict of interest.

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Jiang, D., Jiang, H. & Wang, L. A Novel Paper-Based Capacitance Mast Cell Sensor for Evaluating Peanut Allergen Protein Ara h 2. Food Anal. Methods 13, 1993–2001 (2020). https://doi.org/10.1007/s12161-020-01769-5

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