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Simultaneous Determination of Five Coccidiostats in Veterinary Powders, Feed Premixes, and Baby Food by Micellar Electrokinetic Chromatography: Application to Chicken Tissues and Liver

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Abstract

A new, simple, and reliable micellar electrokinetic chromatographic method was developed and validated for the simultaneous determination of amprolium, ethopabate, diaveridine, sulphadimidine, and sulphaquinoxaline. The separation was achieved using 50 mM tris buffer (pH 8.5) with 50 mM SDS and 15% acetonitrile (v/v) at 28 kV and the components were detected at 200 nm. Different factors affecting the electrophoretic mobility of the five investigated drugs were studied and optimized. Method validation showed that calibration plots were linear within the range from 0.5 to 100 μg/mL with a correlation coefficient > 0.998. Intraday and interday precision and accuracy evaluated by relative standard deviation were lower than 2%. The limits of detection were in the ranges of 0.02 to 0.07 μg/mL. The new method with simple sample pretreatment based on aqueous methanol extraction has been successfully applied for analysis of these drugs in powder preparations, feed premixes, baby food, chicken tissues, and liver samples with the recoveries of 97–101%. The present method is suitable and favorable for the analysis of the five coccidiostats drugs on account of its cost effectiveness, simplicity, rapidity, and sensitivity.

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Acknowledgements

The authors gratefully thank the Alexander von Humboldt Foundation, Bonn, Germany, for donating the instrument (Capillary Electrophoresis device) used in this study to the Faculty of Pharmacy, University of Mansoura.

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Authors and Affiliations

  1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

    Fathalla Belal

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt

    Sawsan Abd El-Razeq & Fatma Fouad

  3. Department of Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, October University for Modern Sciences and Arts, Cairo, Egypt

    Manal Fouad

  4. Unit of Drug Analysis, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

    Sahar Zayed

Authors
  1. Fathalla Belal
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  2. Sawsan Abd El-Razeq
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  3. Manal Fouad
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  4. Sahar Zayed
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  5. Fatma Fouad
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Corresponding author

Correspondence to Sahar Zayed.

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Conflict of Interest

Fathalla Belal declares that he has no conflict of interest. Sawsan Abd El-Razeq declares that she has no conflict of interest. Manal Fouad declares that she has no conflict of interest. Sahar Zayed declares that she has no conflict of interest. Fatma Fouad declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Belal, F., El-Razeq, S.A., Fouad, M. et al. Simultaneous Determination of Five Coccidiostats in Veterinary Powders, Feed Premixes, and Baby Food by Micellar Electrokinetic Chromatography: Application to Chicken Tissues and Liver. Food Anal. Methods 11, 3531–3541 (2018). https://doi.org/10.1007/s12161-018-1330-y

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