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Comparison of Chicken IgY and Mammalian IgG in Three Immunoassays for Detection of Sulfamethazine in Milk

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Abstract

Antibodies are the most important reagents for the development of highly sensitive and specific immunoassays to quantify analytes of interest in food and environmental samples. While immunoglobulin G (IgG)-derived antibodies from rabbit and mouse are traditionally employed in immunoassays, recent findings suggest that chicken egg yolk antibody (immunoglobulin Y (IgY)) provides several advantages over mammalian IgG. However, limited studies to date have examined the possibility of replacing IgG with IgY in immunoassays. In the current investigation, the performance of chicken IgY and IgG derived from rabbit and mouse was systematically compared in terms of sensitivity, specificity, and matrix effect under parallel conditions with three typical assay formats, specifically, indirect competitive enzyme-linked immunosorbent assay (icELISA), fluorescence polarization immunoassay (FPIA), and colloidal gold immunochromatographic assay (GICA), for detection of sulfamethazine (SMZ) as the reference molecule. We evaluated and discussed the influence of different coating antigens, tracers, and physicochemical factors on the performance of IgY and IgG in the immunoassays. Under optimized conditions, the sensitivities of icELISA (IC50 values of 6.70, 4.76, and 1.66 ng mL−1 with recoveries of 86.1–131.8% and precision of < 12%) and FPIA (IC50 values of 24.79, 20.87, and 10.83 ng mL−1 with recoveries of 81.8–120.2% and precision of < 17.3%) based on both IgY and IgG were sufficient to detect SMZ in milk while only GICA based on mouse IgG provided acceptable sensitivity. Our collective data indicate that IgY could be an acceptable alternative to mammalian antibodies in some situations (in icELISA and FPIA) for use in the development of effective immunoassays for screening and detection of veterinary drug residues in food samples.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 31502123 and No. 31601194), China Agriculture Research System (Grant No. CARS-36), Qingdao Science and Technology Project (No. 17-3-3-69-nsh), and Priority Academic Talent Team Cultivation Program of Shandong Colleges and Universities.

Funding

Dr. Xiao Liang and Dr. Wanpeng Yu has received a research grant from the National Natural Science Foundation of China. Prof. Zhanhui Wang has received a research grant from China Agriculture Research System. Dr. Qidi Zhang has received a research grant from Qingdao Science and Technology Project. Dr. Xiao Liang has received a research grant from Priority Academic Talent Team Cultivation Program of Shandong Colleges and Universities.

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Authors and Affiliations

  1. College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, 100193, People’s Republic of China

    Xiao Liang, Yajie Sheng & Zhanhui Wang

  2. College of Veterinary Medicine, Qingdao Agricultural University, No.700 Changcheng Road, Qingdao, 266109, People’s Republic of China

    Xiao Liang, Hu Shan & Qidi Zhang

  3. Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, 266021, People’s Republic of China

    Wanpeng Yu

  4. Department for Safety Supervision of Animal Products, China Animal Health and Epidemiology Center, Qingdao, 266032, China

    Sijun Zhao

Authors
  1. Xiao Liang
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  2. Yajie Sheng
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  3. Wanpeng Yu
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  4. Sijun Zhao
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  5. Hu Shan
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  6. Qidi Zhang
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  7. Zhanhui Wang
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Correspondence to Qidi Zhang or Zhanhui Wang.

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Conflict of Interest

Xiao Liang has no conflict of interest. Yajie Sheng has no conflict of interest. Wanpeng Yu has no conflict of interest. Sijun Zhao has no conflict of interest. Hu Shan has no conflict of interest. Qidi Zhang has no conflict of interest. Zhanhui Wang has no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Informed consent is not applicable in this study.

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Liang, X., Sheng, Y., Yu, W. et al. Comparison of Chicken IgY and Mammalian IgG in Three Immunoassays for Detection of Sulfamethazine in Milk. Food Anal. Methods 11, 3452–3463 (2018). https://doi.org/10.1007/s12161-018-1316-9

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  • DOI: https://doi.org/10.1007/s12161-018-1316-9

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