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Simultaneous Determination of Fluoxastrobin and Tebuconazole in Cucumber and Soil Based on Solid-Phase Extraction and LC-MS/MS Method

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Abstract

Based on aqueous acetonitrile extraction (AAE) and solid-phase extraction (SPE) pretreatments, an analytical method was first introduced for the simultaneous determination of E/Z-fluoxastrobins and tebuconazole in cucumber and soil. The method validation obtained satisfactory results in terms of linearity, matrix effect (− 17.7–2.9%), fortified recovery (78.4–108.0%), precision (1.1–11.9%), and sensitivity (the limits of quantification were 5 μg/kg). During application of the method, the recommended method was used to estimate the dissipation and residues of fluoxastrobin and tebuconazole in cucumber and soil in field tests. The results showed that the half-lives of fluoxastrobin and tebuconazole were 6.5–8.3 days in cucumber and 11.6–12.2 days in soil. In the terminal residue experiment, the residues of fluoxastrobin and tebuconazole were 33–389 and 35–522 μg/kg in cucumber, respectively. Referencing the maximum residue limits (MRLs) of fluoxastrobin (0.5 mg/kg, US Environmental Protection Agency) and tebuconazole (1.0 mg/kg, China), 1 day is recommended as the preharvest interval for the application of fluoxastrobin and tebuconazole in cucumber.

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Funding

This work was funded by the Shanghai Rising-Star Program (17QB1403100), National Key Research and Development Program of China (2016YFD0201009), The National Natural Science Foundation of China (31301699).

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Authors and Affiliations

  1. Pesticide Safety Evaluation Research Center, Institute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 2011106, China

    Weimin Wang, Qiang Sun, Yubo Li, Guangyue Wen, Jiequn Fan, Weiguo Song, Zhihui Zhao & Maofeng Dong

  2. Shanghai Ruifeng Agricultural Sci-Tech Co. Ltd., Shanghai Academy of Agricultural Sciences, Shanghai, 2011106, China

    Maofeng Dong

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  1. Weimin Wang
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  2. Qiang Sun
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  3. Yubo Li
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  7. Zhihui Zhao
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Correspondence to Maofeng Dong.

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Weimin Wang declares that he has no conflict of interest. Qiang Sun declares that he has no conflict of interest. Yubo Li declares that she has no conflict of interest. Guangyue Wen declares that he has no conflict of interest. Jiequn Fan declares that she has no conflict of interest. Weiguo Song declares that he has no conflict of interest. Zhihui Zhao declares that he has no conflict of interest. Maofeng Dong declares that he has no conflict of interest.

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Wang, W., Sun, Q., Li, Y. et al. Simultaneous Determination of Fluoxastrobin and Tebuconazole in Cucumber and Soil Based on Solid-Phase Extraction and LC-MS/MS Method. Food Anal. Methods 11, 750–758 (2018). https://doi.org/10.1007/s12161-017-1044-6

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