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A Simple Validated HPTLC Method for Simultaneous Analysis of Colchicine and Gallic Acid in Polyherbal Formulation: Habb-E-Irqun-Nisha

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Abstract

A simple and affordable high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of colchicine (CL) and gallic acid (GA), which are present together in a complex commercial polyherbal drug (Habb-e-Irqun-Nisa) in tablet dosage forms. The chromatographic separation was carried out on pre-coated silica gel 60 GF254 plates with ethyl acetate/acetonitrile/water/formic acid/N-dimethylformamide 7.5:1:0.5:0.5:0.5 (v/v) as mobile phase. The plates were developed to a distance of 9.0 cm at ambient temperature. The developed plates were scanned and quantified at a single wavelength of maximum absorption at 360 and 287 nm for CL and GA, respectively. Experimental conditions such as band size, chamber saturation time, migration of solvent front, slit width, etc., were critically studied, and the optimum conditions were selected. The drug samples were satisfactorily resolved with Rf 0.35 ± 0.02 for colchicine and 0.69 ± 0.03 for gallic acid. The method was validated for linearity, accuracy, precision and specificity. The calibration plot was linear between 25 and 200 ng per band for CL and 100–800 ng per band for GA. The limits of detection and quantification for CL were 2.0 and 25.00 ng per band, respectively, whereas for GA, these were 10.00 and 100.00 ng per band, respectively. The proposed HPTLC method for estimation of CL and GA was found to be economic, simple, precise, sensitive and less time consuming than other chromatographic procedures and can be used for routine quality control of Habb-e-Irqun-Nisa and other polyherbal formulations containing these phytoconstituents.

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References

  • Emuri A, Lemaire-Hurtel A, DuverneuiU C, Etting I, Guillot E, Mazancourt P, Alvarez TMJ (2006) A novel LC-ESI-MS-MS method for sensitive quantification of colchicine in human plasma: application to two case reports. J Anal Toxicol 30:2010–2015

    Google Scholar 

  • Al-Owaisi M, Al-Hadiwi N, Khan SA (2014) GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves. Asian Pac J Trop Biomed 4:964–970

    Article  CAS  Google Scholar 

  • Anderson, Mark L, David P. Burney (1998) Validation of sample preparation procedures for botanical analysis. J. AOAC Int. 81:1005-1010

  • Bodoki E, Oprean R, Vlase L, Tamas M, Sandulescu R (2005) Fast determination of colchicine by TLC-densitometry from pharmaceuticals and vegetal extracts. J Pharm Biomed Anal 37:971–977

    Article  CAS  Google Scholar 

  • Chan KL, Yuen KH, Jinadasa S, Peh KK, Toh WT (1997) A high-performance liquid chromatography analysis of plasma artemisinin using a glassy carbon electrode for reductive electrochemical detection. Planta Med 63:66–69

    Article  CAS  Google Scholar 

  • Ekor M (2014) The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 4:177 (1-9)

    Article  Google Scholar 

  • European Medicines Agency (2011) EMA/CPMP/QWP/2819/00 Rev. 2. Guideline on quality of herbal medicinal products1 /traditional herbal medicinal products.

  • FDA Guidance for Industry(2004) Botanical drug products. Rochville: US Food and Drug Administration

  • Fernandes FHA, Batista RSA, De Medeiros FD, Santos FS, Medeiros ACD (2015) Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis. Rev Bras 25:208–211

    CAS  Google Scholar 

  • Fernandez P, Bermejo AM, Tabernero MJ, Lopez-rivadulla M, Cruz A (1993) Determination of colchicine in biological fluids by reverse-phase-HPLC. Variation of colchicine levels in rats. Forensic Sci Int 59:15–18

    Article  CAS  Google Scholar 

  • Gong F, Wang BT, Chau FT, Liang YZ (2005) Data pre-processing for chromatographic fingerprint of herbal medicine with chemometric approaches. Anal Lett 38:2475–2492

    Article  CAS  Google Scholar 

  • Hadad GM, Badr JM, El-Nahriry K, Hassanean HA (2013) Validated HPLC and HPTLC methods for simultaneous determination of colchicine and khellin in pharmaceutical formulations. J Chromatogr Sci 51:258–265

    Article  CAS  Google Scholar 

  • Hamscher G, Priess B, Nau H, Panariti E (2005) Determination of colchicine residues in sheep serum and milk using high-performance liquid chromatography combined with electrospray ionization ion trap tandem mass spectrometry. Anal Chem 77:2421–2425

    Article  CAS  Google Scholar 

  • Hostettmann K, Marston A, Maillard M, Hamburger M (eds) (1995) Phytochemistry of plants used in traditional medicine. Clarendon Press, Oxford

    Google Scholar 

  • International Conference on Harmonization (ICH) (2005) Guideline, Q2 (R1), Validation of analytical procedure: text and methodology, in: Proceedings of the International Conference on Harmonization

  • Jiang Y, Wang J, Wang Y, Li H, Fawcett JP, Gu J (2007) Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the quantification of colchicine in human plasma. J Chromatogr B 850:564–568

    Article  CAS  Google Scholar 

  • Kaufman P B, Cseke L J, Warber S, Duke J A, Brielmann H L (1999) Natural products from plants. BocaRaton^ eFL FL: CRC press

  • Khodadoust S, Sadeghi H, Amiri PA, Mohammad J, Salehi A (2015) Optimization of ultrasound-assisted extraction of colchicine compound from Colchicum haussknechtii by using response surface methodology. J Saudi Society Agri Sci. doi:10.1016/j.jssas.2015.05.004

    Google Scholar 

  • Lauer E, Widmer C, Versace F, Staub C, Mangin P, Sabatasso S, Augsburger M, Déglon J (2013) Body fluid and tissue analysis using filter paper sampling support prior to LC-MS/MS: application to fatal overdose with colchicine. Drug Test Anal 5:763–772

    Article  CAS  Google Scholar 

  • Li S, Han Q, Qiao C, Song J, Cheng CL, Xu H (2008) Chemical markers for the quality control of herbal medicines: an overview. Chin Med 3:1–16

    Article  Google Scholar 

  • Muguli G, Rao Vadaparthi PR, Ramesh B, Gowda V, Paramesh R, Jadhav AN, Babu KS (2015) A novel high-performance liquid chromatography-electron spray ionization-mass spectrometry method for simultaneous determination of guggulsterones, piperine and gallic acid in Triphala guggulu. Pharmacogn Mag 11:69–72

    Article  Google Scholar 

  • Put R, Heyden YV (2007) Review on modelling aspects in reversed-phase liquid chromatographic quantitative structure-retention relationships. Anal Chim Acta 602:164–172

    Article  CAS  Google Scholar 

  • Samanidou VF, Sarantis GA, Papadoyannis IN (2006) Development and validation of a rapid HPLC method for the direct determination of colchicine in pharmaceuticals and biological fluids. J Liq Chromatogr Relat Technol 29:1–13

    Article  CAS  Google Scholar 

  • Sheikh Z A, Zahoor A, Shafiq K, Khan S S, Khan A, Usmanghani K (2015) HPTLC methods for the Rapid Determination of Adhatoda vasica L. Glycyrrhiza glabbra L., Phyllanthus embelica L. and Camellia sinensis L. in a polyherbal formulation (INSTY). J. Chem. Soc. Pak 37: 792-801

  • Song R, Xu L, Zhang Z, Tian Y, Xu F, Dong H (2010) Determination of gallic acid in rat plasma by LC-MS-MS. Chromatographia 71:1107–1111

    Article  CAS  Google Scholar 

  • Tian-Ye G, Yan L, Chun-Zhu L, Lin X, Guang-Ji W, Long-Sheng S (2011) Recent development in liquid chromatography/mass spectrometry and allied topics for traditional Chinese medicine research. Chin J Nat Med 9:385–400

    Google Scholar 

  • Tsao R, Yang R (2003) Optimization of a new mobile phase to know the complex and real polyphenolic composition: towards a total phenolic index using high-performance liquid chromatography. J Chromatogr A 1018:29–40

    Article  CAS  Google Scholar 

  • Wang M, Park C, Wu Q, Simon JE (2005) Analysis of artemisinin in Artemisia annua L. by LC-MS with selected ion monitoring. J Agric Food Chem 53:7010–7013

    Article  CAS  Google Scholar 

  • World Health Organization (2008) Traditional medicine. Fact Sheet No. 134. World Health Organization, Geneva

    Google Scholar 

  • Zheng XT, Qu HB (2012) Characterisation of the degradation of salvianolic acid B using an on-line spectroscopic analysis system and multivariate curve resolution. Phytochem Anal 23:103–109

    Article  Google Scholar 

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Acknowledgements

The financial support from the Department of Science and Technology (DST), Ministry of Science and Technology, Government of India, for this work is gratefully acknowledged. The research facilities developed from a UGC-SAP (DRS-1) grant sanctioned to the Department of Biotechnology and used in this study are thankfully acknowledged. The BSR Fellowships awarded under UGC-SAP to A. Ali are also gratefully acknowledged.

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Authors and Affiliations

  1. Centre for Transgenic Plant Development, Department of Biotechnology, Hamdard University, New Delhi, 110062, India

    Athar Ali, Shazia Khan & Malik Zainul Abdin

  2. Quality Control, Hamdard Laboratories, B-1, B-2/III, Industrial Area, Meerut Road, Ghaziabad, 201001, India

    Abdul Qadir

  3. Bond Life Sciences Center, University of Missouri, Columbia, 247, 1201 Rollins Street, Columbia, MO, 65211, USA

    Mather Ali Khan

  4. College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Riyadh, Kingdom of Saudi Arabia

    Faisal Obaid Al-Otaibi

Authors
  1. Athar Ali
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  2. Abdul Qadir
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  3. Mather Ali Khan
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  4. Faisal Obaid Al-Otaibi
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  5. Shazia Khan
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  6. Malik Zainul Abdin
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Correspondence to Malik Zainul Abdin.

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Athar Ali declares that he has no conflict of interest. Abdul Qadir declares that he has no conflict of interest. Mather Ali Khan declares that he has no conflict of interest. Faisal Obaid Al-Otaibi declares that he has no conflict of interest. Shazia Khan declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Ali, A., Qadir, A., Khan, M.A. et al. A Simple Validated HPTLC Method for Simultaneous Analysis of Colchicine and Gallic Acid in Polyherbal Formulation: Habb-E-Irqun-Nisha. Food Anal. Methods 10, 2934–2942 (2017). https://doi.org/10.1007/s12161-017-0835-0

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  • DOI: https://doi.org/10.1007/s12161-017-0835-0

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