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Detailed Profiling of the Volatile Oxygenated Fraction of Mandarin Essential Oils by Using the Off-Line Combination of High-Performance Liquid Chromatography and Comprehensive Two-Dimensional Gas Chromatography-Mass Spectrometry

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Abstract

The aim of the herein described research was the detailed qualitative analysis of the volatile oxygenated fractions of four genuine mandarin (Citrus deliciosa Ten.) essential oils (green, yellow, red, and Mexican), by using the off-line combination of high-performance liquid chromatography (HPLC) and comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (GC×GC-qMS). Detailed qualitative profiling was performed through a pre-separation (normal phase) HPLC process aimed at the isolation of the oxygenated compounds; the target fraction was then concentrated and subjected to cryogenically modulated GC×GC-qMS analysis. The oxygenated constituents contribute most to the essential oil aroma and are also used for the evaluation of quality and genuineness. In such a respect, a deeper analytical view on the qualitative composition of such constituents is certainly useful. In fact, many compounds are herein related to mandarin essential oils for the first time.

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Authors and Affiliations

  1. Chromaleont s.r.l., c/o University of Messina, Polo Annunziata—viale Annunziata, 98168, Messina, Italy

    Mariosimone Zoccali, Paola Dugo, Luigi Mondello & Giovanni Dugo

  2. “Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali”, University of Messina, Polo Annunziata—viale Annunziata, 98168, Messina, Italy

    Peter Q. Tranchida, Ivana L. Bonaccorsi, Paola Dugo, Luigi Mondello & Giovanni Dugo

Authors
  1. Mariosimone Zoccali
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  2. Peter Q. Tranchida
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  3. Ivana L. Bonaccorsi
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  4. Paola Dugo
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  5. Luigi Mondello
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  6. Giovanni Dugo
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Corresponding author

Correspondence to Ivana L. Bonaccorsi.

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Conflict of Interest

Mariosimone Zoccali declares that he has no conflict of interest. Peter Q. Tranchida declares that he has no conflict of interest. Ivana L. Bonaccorsi declares that she has no conflict of interest. Paola Dugo declares that she has no conflict of interest. Luigi Mondello declares that he has no conflict of interest. Giovanni Dugo declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Zoccali, M., Tranchida, P.Q., Bonaccorsi, I.L. et al. Detailed Profiling of the Volatile Oxygenated Fraction of Mandarin Essential Oils by Using the Off-Line Combination of High-Performance Liquid Chromatography and Comprehensive Two-Dimensional Gas Chromatography-Mass Spectrometry. Food Anal. Methods 10, 1106–1116 (2017). https://doi.org/10.1007/s12161-016-0668-2

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  • DOI: https://doi.org/10.1007/s12161-016-0668-2

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