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Preparation of Broadly Specific Monoclonal Antibodies for Simultaneous Determination of Fluoroquinolone Residues in Eggs

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Abstract

To quickly monitor fluoroquinolone (FQ) residues in eggs, three broadly specific monoclonal antibodies (mAbs; i.e., 5B11, 4B6, and 6A2) against FQs were generated. Among them, the 6A2 mAb exhibited the capability to recognize 19 FQs with equal or nearly equal efficacy and therefore was selected for the development of a simple and rapid indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). The optimized ic-ELISA protocol was carried out using only acetonitrile with NaOH for the fast extraction of FQs in whole eggs. The CCα, the CCβ, and the limit of quantity values of 19 FQs in eggs ranged from 0.6 to 3.1, 0.7 to 3.6, and 1.6 to 4.2 μg kg−1, respectively. The recoveries from eggs spiked with the above FQs ranged from 60.2 to 119.9 %, with coefficients of variation (CVs) of less than 17.8 %. These results suggest that the prepared mAbs and the developed ic-ELISA protocol are reliable tools that can be applied for the screening of FQ residues in eggs.

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Authors and Affiliations

  1. National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Dapeng Peng, Yulian Wang, Liang Feng, Guangcai Cao, Yanfei Tao, Zhenli Liu & Zonghui Yuan

Authors
  1. Dapeng Peng
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  2. Yulian Wang
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  3. Liang Feng
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  4. Guangcai Cao
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  5. Yanfei Tao
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  6. Zhenli Liu
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  7. Zonghui Yuan
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Corresponding author

Correspondence to Zonghui Yuan.

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Funding

The work was financially supported by the Ministry of Science and Technology of the People’s Republic of China (2011-G5, GJFP2016008) and the Fundamental Research Funds for the Central Universities (2662015QC030 and 2662015PY021).

Conflict of Interest

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work; there is no professional or other personal interest of any nature or kind in any product, service, and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled “Preparation of broadly specific monoclonal antibodies for simultaneous determination of fluoroquinolone residues in eggs.”

Ethical Approval

This article does not contain any studies with human subjects. All animal experiments that described in the present study were performed in adherence to Huazhong Agricultural University animal experiment center guidelines and approved by Animal Ethics Committee.

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Peng, D., Wang, Y., Feng, L. et al. Preparation of Broadly Specific Monoclonal Antibodies for Simultaneous Determination of Fluoroquinolone Residues in Eggs. Food Anal. Methods 9, 3520–3531 (2016). https://doi.org/10.1007/s12161-016-0546-y

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  • DOI: https://doi.org/10.1007/s12161-016-0546-y

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