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Capillary Isoelectric Focusing—Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk

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Abstract

The lactic acid bacteria (LAB), including lactobacilli and enterococci, represent an important part of normal microflora in humans. Simultaneously, they are frequently used as probiotics and in food industry for production of fermented milk products. The rapid and sensitive detection of these microorganisms is crucial for the quality control. In this study, the capillary isoelectric focusing (CIEF) was successfully used for the separation and characterization of probiotic LAB, L. paracasei sp. paracasei; L. acidifarinae; L. fermentum; L. gasseri; L. helveticus; L. plantarum; L. delbrueckii; L. salivarius; E. durans; E. faecalis; and E. faecium, according to their isoelectric points (pI). All pIs of lactobacilli and enterococci were found in the acidic part of the pH range. Subsequently, the milk samples spiked with L. rhamnosus, and E. faecium were analyzed by CIEF. The optimal adjustment of the separation conditions allowed detection and quantification of the bacteria in the sample with sufficient sensitivity. Therefore, CIEF is an efficient approach to rapid detection and separation of LAB even directly in milk products in future.

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Funding

This study was funded by the Ministry of Agriculture of the Czech Republic (projects QI QI91B274 and QI111B053), by the Academy of Sciences of the Czech Republic (Institutional Support RVO: 68081715), and by the Ministry of Health of the Czech Republic (16-29916A, 16-31593A).

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Authors and Affiliations

  1. The Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 53/5, 625 00, Brno, Czech Republic

    F. Ruzicka, V. Hola & K. Mlynarikova

  2. The Department of Microbiology, St. Anne’s University Hospital, Brno, Pekařská 53, 602 00, Brno, Czech Republic

    F. Ruzicka & V. Hola

  3. Institute of Analytical Chemistry of the CAS, v. v. i., Veveří 97, 602 00, Brno, Czech Republic

    M. Horka

  4. Dairy Research Institute, Tabor, Czech Republic

    V. Drab

Authors
  1. F. Ruzicka
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  2. M. Horka
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  3. V. Hola
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  4. K. Mlynarikova
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  5. V. Drab
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Correspondence to F. Ruzicka.

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Conflict of Interest

F. Ruzicka, M. Horka, V. Hola, K. Mlynarikova, and V. Drab declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Ruzicka, F., Horka, M., Hola, V. et al. Capillary Isoelectric Focusing—Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk. Food Anal. Methods 9, 3251–3257 (2016). https://doi.org/10.1007/s12161-016-0522-6

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  • DOI: https://doi.org/10.1007/s12161-016-0522-6

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