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A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples

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Abstract

This paper describes the development of a rapid and clean method for the determination of hydrolysable tannins in honey samples, focusing on an easy and green sample pretreatment and low generation of waste. The proposed method is based on the reaction between KIO3 and hydrolysable tannin compounds in acetate buffer medium (pH 4.75), with formation of a colored product measured at 525 nm. The analytical conditions were optimized using experimental design (central composite design). The linear range obtained was 40.0–740 mg L−1 (r = 0.998), and the detection (LOD) and quantification (LOQ) limits were 12.2 and 40.8 mg L−1, respectively. Acetate buffer was used to ensure formation of the colored product obtained by reaction between the chromogenic reagent and the analyte. The method showed good results when applied to honey samples, with recoveries in the range of 78.3–96.1 %, and the use of diffuse reflectance spectroscopy coupled with spot test analysis provided low reagent consumption and minimized waste generation. The results obtained with the proposed method were confirmed by a spectrophotometric method.

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Acknowledgments

We would like to thank CNPq for the financial support and for a scholarship awarded to T. A. Catelani.

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Authors and Affiliations

  1. Instituto de Química, Universidade Estadual Paulista “Julio de Mesquita Filho”, UNESP, R. Prof. Francisco Degni 55, P.O. Box 355, 14800-900, Araraquara, SP, Brazil

    Tiago Augusto Catelani, Dayana Borges Bittar, Leonardo Pezza & Helena Redigolo Pezza

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  1. Tiago Augusto Catelani
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  2. Dayana Borges Bittar
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  3. Leonardo Pezza
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  4. Helena Redigolo Pezza
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Correspondence to Helena Redigolo Pezza.

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On behalf of all the authors of this original research paper, the author Helena Redigolo Pezza declares that this article does not contain any studies involving human participants or animals performed by any of the authors.

Funding

This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasília, Brazil.

Conflict of Interest

Helena Redigolo Pezza declares that he has no conflict of interest. Tiago Augusto Catelani declares that he has no conflict of interest. Dayana Borges Bittar declares that he has no conflict of interest. Leonardo Pezza declares that he has no conflict of interest.

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Catelani, T.A., Bittar, D.B., Pezza, L. et al. A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples. Food Anal. Methods 9, 2552–2559 (2016). https://doi.org/10.1007/s12161-016-0454-1

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  • DOI: https://doi.org/10.1007/s12161-016-0454-1

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