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Components Analysis of Recycled Alkali Black Liquor Combined with Corn Straw Under Ozone Pretreatment

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Abstract

Alkali combined with ozone pretreatment of corn straw could improve the enzymatic hydrolysis degree of cellulose to realize the biomass conversion of straw waste. In order to recycle the alkali black liquor derived from this process and realize the environment-friendly effect, the main components of alkali combined with ozone pretreatment of straw recycling black liquor were investigated in this paper. To this end, the alkali black liquor was sequentially withdrawn for 0–6 times under the optimal pretreatment conditions, and solid content, organic and inorganic matter, acid precipitation, alkali precipitation, and organic composition were analyzed to identify the main factors inhibiting cellulase hydrolysis in recycled alkali black liquor. The results showed that the cellulase hydrolysis rate presented a significant decrease at the fourth cycle, which was decreased by 11.39%, and the content of alkali precipitation was maximum (0.707 g). Meanwhile, the organic matter content in alkali precipitation also reached the maximum value of 0.491 g. Through the component analysis, the contents of lignin and acid precipitation increased throughout the cycles. Moreover, GC–MS results showed that phenols, benzene ring heterocyclic, and furans were main degradation products in cycles of black liquor. Accordingly, it was indicated that small molecular organics and lignin were inhibitors of cellulase hydrolysis, which accumulated during recycling, and reduced alkali utilization and delignification efficiency, resulting in lower enzymatic hydrolysis rate.

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References

  1. Mathew AK, Parameshwaran B, Sukumaran RK, Pandey A (2016) An evaluation of dilute acid and ammonia fiber explosion pretreatment for cellulosic ethanol production. Bioresour Technol 199:13–20. https://doi.org/10.1016/j.biortech.2015.08.121

    Article  CAS  PubMed  Google Scholar 

  2. Ravindran R, Jaiswal AK (2016) A comprehensive review on pre-treatment strategy for lignocellulosic food industry waste: challenges and opportunities. Bioresour Technol 199:92–102. https://doi.org/10.1016/j.biortech.2015.07.106

    Article  CAS  PubMed  Google Scholar 

  3. Muryanto TE, Hendarsyah H, Abimanyu H (2015) Reuse black liquor of alkali pretreatment in bioethanol production. Energy Procedia 68:236–243. https://doi.org/10.1016/j.egypro.2015.03.252

    Article  CAS  Google Scholar 

  4. Sivagurunathan P, Kumar G, Mudhoo A, Rene ER, Saratale GD, Kobayashi T, Xu K, Kim S, Kim D (2017) Fermentative hydrogen production using lignocellulose biomass: an overview of pre-treatment methods, inhibitor effects and detoxification experiences. Renew Sustain Energy Rev 77:28–42. https://doi.org/10.1016/j.rser.2017.03.091

    Article  CAS  Google Scholar 

  5. Saha BC, Qureshi N, Kennedy GJ, Cotta MA (2016) Biological pretreatment of corn stover with white-rot fungus for improved enzymatic hydrolysis. Int Biodeter Biodegr 109:29–35. https://doi.org/10.1016/j.ibiod.2015.12.020

    Article  CAS  Google Scholar 

  6. Brahim M, Fernandez BLC, Regnier O, Boussetta N, Grimi N, Sarazin C, Husson E, Vorobiev E, Brosse N (2017) Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw’s lignin and pulps. Bioresour Technol 237:11–19. https://doi.org/10.1016/j.biortech.2017.04.003

    Article  CAS  PubMed  Google Scholar 

  7. Pielhop T, Amgarten J, von Rohr PR, Studer MH (2016) Steam explosion pretreatment of softwood: the effect of the explosive decompression on enzymatic digestibility. Biotechnol Biofuels 9https://doi.org/10.1186/s13068-016-0567-1

  8. Wang Z, Zhu M, Li M, Wei Q, Sun R (2019) Effects of hydrothermal treatment on enhancing enzymatic hydrolysis of rapeseed straw. Renew Energy 134:446–452. https://doi.org/10.1016/j.renene.2018.11.019

    Article  CAS  Google Scholar 

  9. Damaurai J, Champreda V, Laosiripojana N (2014) Optimization of alkaline peroxide pretreatment of rice straw. J Nat Sci Res 4:29–33

    Google Scholar 

  10. Chiranjeevi T, Mattam AJ, Vishwakarma KK, Uma A, Peddy VCR, Gandham S, Velankar HR (2018) Assisted single-step acid pretreatment process for enhanced delignification of rice straw for bioethanol production. ACS Sustain Chem Eng 6:8762–8774. https://doi.org/10.1021/acssuschemeng.8b01113

    Article  CAS  Google Scholar 

  11. Kim JS, Lee YY, Kim TH (2016) A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresour Technol 199:42–48. https://doi.org/10.1016/j.biortech.2015.08.085

    Article  CAS  PubMed  Google Scholar 

  12. Goshadrou A (2019) Bioethanol production from Cogongrass by sequential recycling of black liquor and wastewater in a mild-alkali pretreatment. Fuel 258https://doi.org/10.1016/j.fuel.2019.116141

  13. Wang P, Liu C, Chang J, Yin Q, Huang W, Liu Y, Dang X, Gao T, Lu F (2019) Effect of physicochemical pretreatments plus enzymatic hydrolysis on the composition and morphologic structure of corn straw. Renew Energy 138:502–508. https://doi.org/10.1016/j.renene.2019.01.118

    Article  CAS  Google Scholar 

  14. Jiang B, Zhang Y, Gu L, Wu W, Zhao H, Jin Y (2018) Structural elucidation and antioxidant activity of lignin isolated from rice straw and alkali oxygen black liquor. Int J Biol Macromol 116:513–519. https://doi.org/10.1016/j.ijbiomac.2018.05.063

    Article  CAS  PubMed  Google Scholar 

  15. Fang S, Wang W, Tong S, Zhang C, Liu P (2018) Evaluation of the effects of isolated lignin on cellulose enzymatic hydrolysis of corn stover pretreatment by NaOH combined with ozone. Molecules 23https://doi.org/10.3390/molecules23061495

  16. Zhou X, Wang M, Fang S, Liu X, Liu P (2019) Effect of alkaline black liquor recycling on alkali combined with ozone pretreatment of corn stalk. Molecules 24:2836. https://doi.org/10.3390/molecules24152836

    Article  CAS  PubMed Central  Google Scholar 

  17. Cha Y, Yang J, Seo S, An GH, Moon Y, You G, Lee J, Ahn J, Lee K (2016) Alkaline twin-screw extrusion pretreatment of Miscanthus with recycled black liquor at the pilot scale. Fuel 164:322–328. https://doi.org/10.1016/j.fuel.2015.10.006

    Article  CAS  Google Scholar 

  18. Chandel AK, Da Silva SS, Singh OV (2013) Detoxification of lignocellulose hydrolysates: biochemical and metabolic engineering toward white biotechnology. Bioenergy Res 6:388–401. https://doi.org/10.1007/s12155-012-9241-z

    Article  CAS  Google Scholar 

  19. Panagiotou G, Olsson L (2007) Effect of compounds released during pretreatment of wheat straw on microbial growth and enzymatic hydrolysis rates. Biotechnol Bioeng 96:250–258. https://doi.org/10.1002/bit.21100

    Article  CAS  PubMed  Google Scholar 

  20. Ximenes E, Kim Y, Mosier N, Dien B, Ladisch M (2010) Inhibition of cellulases by phenols. Enzyme Microb Technol 46:170–176. https://doi.org/10.1016/j.enzmictec.2009.11.001

    Article  CAS  Google Scholar 

  21. Sluiter A, Hames B, Ruiz RO, Scarlata C, Sluiter J, Templeton D (2008) Determination of structural carbohydrates and lignin in biomass: laboratory analytical procedure (LAP) national renewable energy laboratory: Golden, CO, USA

  22. Wang W, Zhang C, Tong S, Cui Z, Liu P (2018) Enhanced enzymatic hydrolysis and structural features of corn stover by NaOH and ozone combined pretreatment. Molecules 23:1300. https://doi.org/10.3390/molecules23061300

    Article  CAS  PubMed Central  Google Scholar 

  23. Liu XM, Zhang JY, Zhang B, Liu XY, Chen XF (2016) Study on the composition and evaporation characteristics of pulping black liquor. Transactions of China Pulp and Paper, 33: 35-39

  24. Rong SP, Sun YB, Zhao ZH (2013) Comparative study on the treatment of straw pulping black liquid by acid-precipitation and alkali-precipitation. Environ Prot Chem Indus 33:221–225

    CAS  Google Scholar 

  25. Li Y, Fang S, Zhou X, Zhao Z, Li F, Liu P (2020) Adsorption study of lignin removal from recycled alkali black liquor by adsorption resins for improved cellulase hydrolysis of corn straw. Molecules 25:4475. https://doi.org/10.3390/molecules25194475

    Article  CAS  PubMed Central  Google Scholar 

  26. Yang B, Wyman CE (2010) Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol Bioeng 86:88–98. https://doi.org/10.1002/bit.20043

    Article  CAS  Google Scholar 

  27. Liu L, Sun J, Min L, Wang S, Pei H, Zhang J (2009) Enhanced enzymatic hydrolysis and structural features of corn stover by FeCl3 pretreatment. Bioresour Technol 100:5853–5858. https://doi.org/10.1016/j.biortech.2009.06.040

    Article  CAS  PubMed  Google Scholar 

  28. Guo DL, Wu SB, Zhao YS, Dai T, Yin XL (2012) Catalytic effects of existing form of Na atom on alkali lignin pyrolysis. Proceeding of the 4th International Conference on Pulping, Papermaking and Biotechnology.

  29. Moraes Rocha GJ, Nascimento VM, Nunes Da Silva VF, Simoes Corso DL, Goncalves AR (2014) Contributing to the environmental sustainability of the second generation ethanol production: delignification of sugarcane bagasse with sodium hydroxide recycling. Ind Crop Prod 59:63–68. https://doi.org/10.1016/j.indcrop.2014.05.002

    Article  CAS  Google Scholar 

  30. Brebu M, Tamminen T, Spiridon I (2013) Thermal degradation of various lignins by TG-MS/FTIR and Py-GC-MS. J Anal Appl Pyrolysis 104:531–539. https://doi.org/10.1016/j.jaap.2013.05.016

    Article  CAS  Google Scholar 

  31. Ma Z, Sun Q, Ye J, Yao Q, Zhao C (2016) Study on the thermal degradation behaviors and kinetics of alkali lignin for production of phenolic-rich bio-oil using TGA-FTIR and Py-GC/MS. J Anal Appl Pyrolysis 117:116–124. https://doi.org/10.1016/j.jaap.2015.12.007

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China

    Xiaohong Lu, Fei Li, Yiming Li, Shuo Fang, Xia Zhou, Zhezhen Zhao & Ping Liu

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  1. Xiaohong Lu
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  2. Fei Li
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  3. Yiming Li
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  4. Shuo Fang
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  5. Xia Zhou
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  6. Zhezhen Zhao
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  7. Ping Liu
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Contributions

Conceptualization: Yiming Li and Shuo Fang; methodology: Yiming Li, Shuo Fang and Xia Zhou; software: Yiming Li and Shuo Fang; visualization: Shuo Fang; formal analysis: Fei Li, Xiaohong Lu and Yiming Li; writing—original draft: Yiming Li, Fei Li and Xiaohong Lu; writing—review and editing: Fei Li, Xiaohong Lu, Xia Zhou, Zhezhen Zhao, and Ping Liu; Supervision: Ping Liu. All authors contributed to this work by collaboration.

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Correspondence to Ping Liu.

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Xiaohong Lu, Fei Li, and Yiming Li contributed equally to this work.

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Lu, X., Li, F., Li, Y. et al. Components Analysis of Recycled Alkali Black Liquor Combined with Corn Straw Under Ozone Pretreatment. Bioenerg. Res. 15, 1204–1212 (2022). https://doi.org/10.1007/s12155-021-10354-5

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