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FAIMS-MS-IR spectroscopy workflow: a multidimensional platform for the analysis of molecular isoforms

  • Original Research
  • Published:
International Journal for Ion Mobility Spectrometry

Abstract

An original workflow allowing inline FAIMS separation, electrospray ionization, mass analysis and ion spectroscopy (IRMPD: InfraRed Multiple Photon Dissociation) is presented for multidimensional molecular analysis. This new instrument consists of an ultraFAIMS (Owlstone) device interfaced to a linear ion trap (LTQ XL Thermo Scientific) which was modified for IRMPD spectroscopy. Two modes of operation are demonstrated on an isomeric mixture of paracetamol and 2-phenylglycine. In the first mode a FAIMS (high-Field Asymmetric waveform Ion Mobility Spectrometry) separation of the isomers is performed with a static compensation field for mass- and isomer- selective ion spectroscopy. In the second mode, the compensation field is scanned while the ions are irradiated at a fixed wavenumber. The advantages of this workflow as compared to traditional FAIMS-MS and IRMPD spectroscopy are described. The potential of the two modes for molecular spectroscopy and analytical applications, in particular the new “omics” are discussed.

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Acknowledgements

This work was supported by Institut Universitaire de France, ANR Circé (grant ANR-16-CE30-0012) the Fédération de Recherche André Marie Ampère and the Glycophysics Network (web: http://glyms.univ-lyon1.fr) funded by the French Agence Nationale de la Recherche (grant ANR-2015-MRSEI-0010).

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Authors and Affiliations

  1. CNRS, Institut Lumière Matière, Univ Lyon, Université Claude Bernard Lyon 1, F-69622, Villeurbanne, France

    Baptiste Schindler, Agathe Depraz Depland, Gina Renois-Predelus, Gabriel Karras, Bruno Concina, Gulabi Celep, Jacques Maurelli, Vincent Loriot, Eric Constant, Richard Bredy, Christian Bordas, Franck Lépine & Isabelle Compagnon

  2. Institut Universitaire de France IUF, 103 Boulevard St Michel, 75005, Paris, France

    Isabelle Compagnon

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  1. Baptiste Schindler
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  2. Agathe Depraz Depland
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  3. Gina Renois-Predelus
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  4. Gabriel Karras
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  13. Isabelle Compagnon
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Correspondence to Baptiste Schindler or Isabelle Compagnon.

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Schindler, B., Depland, A.D., Renois-Predelus, G. et al. FAIMS-MS-IR spectroscopy workflow: a multidimensional platform for the analysis of molecular isoforms. Int. J. Ion Mobil. Spec. 20, 119–124 (2017). https://doi.org/10.1007/s12127-017-0225-8

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  • DOI: https://doi.org/10.1007/s12127-017-0225-8

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