Abstract
The N-terminal half of La protein consists of two concatenated motifs: La motif (LAM) and the N-terminal RNA recognition motif (RRM1) both of which are responsible for poly(U) RNA binding. Here, we present the backbone and side-chain assignments of the 1H, 13C and 15N resonances of the 191-residue LAM–RRM1 region of the La protein from the lower eukaryote Dictyostelium discoideum and its secondary structure prediction.
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Abbreviations
- NMR:
-
Nuclear magnetic resonance
- HSQC:
-
Heteronuclear single quantum coherence
- LAM:
-
La motif
- RRM:
-
RNA recognition motif
- RRM1:
-
N-terminal RNA recognition motif
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Acknowledgments
The authors thank Prof. D. Drainas for kindly providing the cDNA library. ‘‘SEE-DRUG’’ Grant (EU FP7 REGPOT CT-2011-285950; www.seedrug.upatras.gr) and ‘‘K. Karatheodori’’ Grant 2010 (D.164; UPAT Research Committee to C.S.) are acknowledged for financial support. This work was additionally supported in part and implemented under the ‘‘ARISTEIA’’ action of the ‘‘OPERATIONAL PROGRAMME EDUCATION AND LIFELONG LEARNING’’ and is co-funded by the European Social Fund (ESF) and National Resources (MIS 1225, No. D608). D.B. thanks Dr. B. Fakler (University of Freiburg) for continued support.
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Christos T. Chasapis and Aikaterini I. Argyriou have contributed equally to this work.
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Chasapis, C.T., Argyriou, A.I., Apostolidi, M. et al. 1H, 13C and 15N backbone and side-chain resonance assignment of the LAM–RRM1 N-terminal module of La protein from Dictyostelium discoideum . Biomol NMR Assign 9, 303–307 (2015). https://doi.org/10.1007/s12104-015-9597-z
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DOI: https://doi.org/10.1007/s12104-015-9597-z