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1,25(OH)2 vitamin D3 signalling on immature rat Sertoli cells: gamma-glutamyl transpeptidase and glucose metabolism

  • RESEARCH ARTICLE
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Journal of Cell Communication and Signaling Aims and scope

Abstract

1α,25-Dihydroxyvitamin D3 (1,25-D3) is critical for the maintenance of normal male reproduction since reduced fertility is observed in vitamin D-deficient rats. Gamma-glutamyl transpeptidase (GGT) is a membrane-bound enzyme that is localized on Sertoli cells and catalyses the transfer of the gamma-glutamyl residues to an amino acid or peptide acceptor. Sertoli cells are also responsible for providing nutrients, as lactate, to the development of germ cells. The aim of this study was to investigate the effect and the mechanism of action of 1,25-D3 on GGT on Sertoli cell functions from 30-day-old immature rat testis. Results demonstrated that 1,25-D3 stimulates GGT activity at Sertoli cells plasma membrane through a PKA-dependent mechanism of action, which was not dependent of active de novo protein synthesis. The hormone increases glucose uptake, as well as lactate production and release by Sertoli cells without altering the reactive oxygen species (ROS) generation. In addition, 1,25-D3 did not change reduced glutathione (GSH) amount or oxygen consumption, and diminished Sertoli cell death. These findings demonstrate that 1,25-D3 stimulatory effect on GGT activity, glucose uptake, LDH activity and lactate production seem to be an important contribution of Sertoli cells for germ cells nutrition and for a full and active ongoing spermatogenesis.

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Abbreviations

BDC:

Bisdemethoxycurcumin

CM:

Curcumin

1,25-D3 :

1α,25-dihydroxyvitamin D3

14C–DG:

[U-14C]-2-deoxy-D-glucose

GGT:

Gamma-glutamyl transpeptidase

KRb:

Krebs Ringer-bicarbonate buffer

LDH:

Lactate dehydrogenase

GSH:

Reduced gluthatione

VDR:

Vitamin D receptor

VDR-AP:

Vitamin D alternative pocket

VDRnuc:

Vitamin D nuclear receptor

VDRmem:

Vitamin D membrane receptor

CH:

Cycloheximide

ST:

Stearoylcarnitine chloride

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Acknowledgments

This study was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico-Brasil (CNPq n° 472071/2013-0) and Coordenação de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado de Santa Catarina (FAPESC). RG, AR and DLS are registered on the PPG-Biochemistry/UFSC. APZ is registered on the PGFAR/UFSC. FRMBS, AZ and AL are recipient of CNPq productivity fellowship. We thank LAMEB I-CCB/UFSC technicians for their support.

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  1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx Postal 5069, Florianópolis, Santa Catarina, CEP: 88040-970, Brazil

    Renata Gonçalves, Ariane Zamoner, Ana Paula Zanatta, Aline Pertile Remor, Débora da Luz Scheffer, Alexandra Latini & Fátima Regina Mena Barreto Silva

  2. Departamento de Enfermagem, Centro de Educação Superior do Oeste, Universidade do Estado de Santa Catarina, Chapecó, Santa Catarina, Brazil

    Leila Zanatta

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  1. Renata Gonçalves
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  2. Ariane Zamoner
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  8. Fátima Regina Mena Barreto Silva
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Correspondence to Fátima Regina Mena Barreto Silva.

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Gonçalves, R., Zamoner, A., Zanatta, L. et al. 1,25(OH)2 vitamin D3 signalling on immature rat Sertoli cells: gamma-glutamyl transpeptidase and glucose metabolism. J. Cell Commun. Signal. 11, 233–243 (2017). https://doi.org/10.1007/s12079-016-0367-1

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