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Association of adiponectin gene polymorphisms and additional gene-gene interaction with nonalcoholic fatty liver disease in the Chinese Han population

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Abstract

Purpose

To investigate the association between the single nucleotide polymorphisms (SNPs) of the adiponectin gene and nonalcoholic fatty liver disease (NAFLD) as well as the impact of the interaction of multiple SNPs on NAFLD risk, based on a Chinese population study.

Methods

A total of 612 subjects (411 male, 201 female) were selected, including 302 NAFLD patients and 310 controls. Three SNPs were selected for genotyping in the case-control study: rs266729, rs822393, and rs1501299. A logistic regression model was used to examine the interaction between the SNPs and NAFLD. The odds ratio (OR) and 95 % confidence interval (95 % CI) were calculated. Generalized multifactor dimensionality reduction (GMDR) was employed to analyze the interaction among SNPs.

Results

Logistic analysis showed a significant association between genotypes of variants in rs266729 and rs822393 and increased NAFLD risk. The carriers of the homozygous mutant of two SNP polymorphisms revealed increased NAFLD risk compared to those with wild-type homozygotes; ORs (95 % CI) were 1.31 (1.14–1.81) (p = 0.001) and 1.18 (1.05–1.71) (p = 0.005), respectively. There was a significant two-locus model (p = 0.0010) involving rs266729 and rs822393, indicating a potential gene-gene interaction between rs266729 and rs822393. Overall, the two-locus models had a cross-validation consistency of 10 and testing accuracy of 62.17 %. Subjects with the CG or GG and CT or TT genotype have the highest NAFLD risk compared to subjects with the CC-CC genotype; the OR (95 % CI) was 2.52 (1.31–3.82), p < 0.001, after covariate adjustment.

Conclusions

Our results support an important association of the rs266729 (−11377 G/C) and rs822393 (−4522 C/T) polymorphism with increased risk of NAFLD. The interaction analysis showed a combined effect of rs266729 and rs822393 on NAFLD.

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Acknowledgements

The writing of this article was supported by the Affiliated Hospital of Jiangsu University. We thank all the partners and staff who helped us in the process of this study.

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Authors and Affiliations

  1. Department of Gastroenterology, Affiliated Hospital of Jiangsu University, No. 308 Jiefang Road, Zhenjiang, Jiangsu, 212001, China

    Zhang Wei & Zhu Li-Qun

  2. The Hospital of the 4th Division, Department of Gastroenterology, Xinjiang Production and Construction Corps, Yi’ning, 835000, China

    Huo Xiao-Ling & Qin Jian

  3. Department of Endocrinology, The Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China

    Yuan Guo-Yue

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  1. Zhang Wei
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  2. Zhu Li-Qun
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Correspondence to Zhang Wei.

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Funding

This work was supported by Social Development of Science and Technology Support Projects, Zhenjiang (grant no. SH2013036), and Science and Technology Assistance Projects, Xinjiang (grant no. 2014AB045).

Conflict of interest

Zhang Wei, Zhu Li-Qun, Huo Xiao-Ling, Qin Jian, and Yuan Guo-Yue declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Blood samples were collected from each participant. Written informed consent was obtained from each individual prior to participation in the study.

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Wei, Z., Li-Qun, Z., Xiao-Ling, H. et al. Association of adiponectin gene polymorphisms and additional gene-gene interaction with nonalcoholic fatty liver disease in the Chinese Han population. Hepatol Int 10, 511–517 (2016). https://doi.org/10.1007/s12072-015-9687-0

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  • DOI: https://doi.org/10.1007/s12072-015-9687-0

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