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Expression Levels of Glutathione S-Transferase and Cytochrome P450 Isoenzymes in Nasal Polyp Tissue

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Abstract

Nasal polyps are benign sinonasal masses composed of eosinophils and extracellular edema. Pathogenesis of the polyp formation is unclear but several studies strongly suggest a correlation with infection, inflammation and allergy conditions. Our aim is to investigate the potential link between allergy and nasal polyp in tissue level. Nasal polyp group included 60 patients whose diagnosis was confirmed with biopsy and the control group included 38 healthy patients. Tissue sample of the control group was taken from inferior turbinate mucosa under local anesthesia and nasal polyp tissue was collected from functional endoscopic sinus surgery. The glutathione S-transferase (GST) and cytochrome P450 (CYP) isoenzyme expressions of the tissue samples were investigated under light microscopy and graded by a senior pathologist. GSTP1 protein expression was significantly higher in tissue samples from nasal polyp group compared to that of control group (p < 0.05). However, CYP1A1, GSTM1 and GSTA1 isoenzymes were not different between the two groups (p > 0.05). We have found that GSTP1 isoenzyme was elevated in nasal polyp tissue compared to the control. The increase in protein expression of GSTP1 might have occured as a tissue response to the increased oxidative stress thus suggesting a role of GSTP1 in polyp formation.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Istanbul Medical School, Istanbul University, Istanbul, Turkey

    Mehmet Gökhan Demir & Sedat Aydın

  2. Biology Department, Faculty of Arts and Sciences, Kırıkkale University, Kirikkale, Turkey

    Serpil Oğuztüzün, Büşra M. Bozer & Murat Kılıç

  3. Pathology Department, Kartal Dr. Lutfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

    Kayhan Başak

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  1. Mehmet Gökhan Demir
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  2. Sedat Aydın
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Contributions

All the authors contribute all the process of the manuscript preperation. Conceptualization, MGD, SA, SO and KB.; methodology, MGD, SA, SO and KB.; software, MGD, SA, SO and KB.; validation, MGD, SA, SO and KB.; formal analysis, MGD, SA, SO and KB.; investigation, MGD, SA, SO, BMB, MK and KB.; resources, MGD, SA, SO, MK, BMB and KB.; data curation, MGD, SA, SO and KB.; writing—original draft preparation, MGD, SA, SO, MK and KB.; writing—review and editing, MGD, SA, SO and KB; visualization, MGD, SA, SO and KB.; supervision, MGD, SA, SO and KB.; project administration, MGD, SA, SO and KB.; funding acquisition, MGD, SA, SO and KB. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Mehmet Gökhan Demir.

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Demir, M.G., Aydın, S., Oğuztüzün, S. et al. Expression Levels of Glutathione S-Transferase and Cytochrome P450 Isoenzymes in Nasal Polyp Tissue. Indian J Otolaryngol Head Neck Surg 75, 178–182 (2023). https://doi.org/10.1007/s12070-022-03353-1

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