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Genetic characterization of maize doubled haploid lines for Fusarium stalk rot caused by Fusarium verticillioides

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Abstract

Fusarium stalk rot disease (FSR) of maize caused by Fusarium verticillioides (Sacc.) Nirenberg is becoming an important biotic production constraint in many of the major maize growing areas causing substantial yield losses. Inbreds are preferred as parents in hybrid development owing to homozygous nature and high heterotic ability. Double haploid (DH) technology has emerged as a significant milestone. A total of 339 DH lines were generated from two inbred lines, VL1043 (susceptible) and CM212 (resistant), through in vivo haploid induction method. The 339 DH lines along with parents were phenotyped for their response to the FSR at the College of Agriculture, V. C. Farm, Mandya, India during summer, kharif and rabi seasons of the 2019–2020. Best linear unbiased predictors (BLUPs) were estimated for the FSR disease scores over three seasons. A wide range of BLUP scores of three to nine indicated the presence of higher variation for response of DH lines to FSR disease. The higher estimates of standardized range (1.31) and phenotypic coefficient of variation (19.80) also displayed higher variability. Nine lines were moderately resistant and 188 exhibited moderately susceptible reaction. The distribution of DH lines was positively skewed (1.34) and platykurtic (2.31) which suggested complementary epistasis and involvement of large number of genes in the disease expression.

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Acknowledgements

Authors are thankful to the Directorate of Research, UAS, Bengaluru, India, for adequate funding and M/s Corteva Agriscience Pvt. Ltd., Gouribidanur, for generating doubled haploid lines of maize which were used in the present study. The first author acknowledges the Directorate of Minorities, Government of Karnataka, India, for providing scholarship during the course of study and to pursue the Ph.D. programme.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India

    B. M. Showkath Babu

  2. Department of Genetics and Plant Breeding, College of Agriculture, V. C. Farm, Mandya, 571 405, India

    H. C. Lohithaswa

  3. AICRP on Maize, Zonal Agricultural Research Station, V. C. Farm, Mandya, 571 405, India

    N. Mallikarjuna

  4. Corteva Agriscience Pvt. Ltd, Research Farm, Kallinayakanahalli, Gauribidanur Taluk, Chikkaballapur, India

    Anand Pandravada & D. C. Balasundara

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  1. B. M. Showkath Babu
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  2. H. C. Lohithaswa
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  3. N. Mallikarjuna
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  4. Anand Pandravada
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  5. D. C. Balasundara
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Correspondence to H. C. Lohithaswa.

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Corresponding editor: Manoj Prasad

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Showkath Babu, B.M., Lohithaswa, H.C., Mallikarjuna, N. et al. Genetic characterization of maize doubled haploid lines for Fusarium stalk rot caused by Fusarium verticillioides. J Genet 99, 83 (2020). https://doi.org/10.1007/s12041-020-01236-4

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  • DOI: https://doi.org/10.1007/s12041-020-01236-4

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