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Molecular cloning and mRNA expression pattern of \(\varvec{Sox}\)4 in Misgurnus anguillicaudatus

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Abstract

Sox4, a member of the SoxC subfamily which of the Sox family, plays important roles in the development of the vertebrate gonad and nervous system. We have cloned a Sox4 homologue from the brain of Misgurnus anguillicaudatus using homologous cloning and rapid amplification of cDNA ends. We named the cloned gene as MaSox4. The full-length cDNA was 2122 bp, containing a 718 bp \(5^{\prime }\)-untranslated region and a 267 bp \(3^{\prime }\)-untranslated region. The open-reading frame of the cloned gene encoded 378 amino acids and contained a characteristic HMG-box DNA-binding domain with the specific motif (RPMNAFMVW). Phylogenetic analysis indicated that MaSox4 is highly homologous to Sox4 in different species. Protein sequence analysis showed that MaSox4 is a nonsecretory hydrophilic protein. Quantitative real-time reverse transcription polymerase chain reaction and in situ hybridization assay revealed that MaSox4 was ubiquitously expressed during embryogenesis and is present in various adult tissues, especially in the central nervous system. Our study suggests that MaSox4 is highly conserved among vertebrates’ evolution and might be involved in developmental processes such as embryogenesis, neurogenesis and gonad development.

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Acknowledgements

This work is supported by grants from the National Natural Science Foundation of China (no. 31200923), the Young Core Instructor Foundation of Henan Normal University (no. 5101049470610), the Doctor Subject Foundation of Henan Normal University (no. 01046500109) and Programme for Innovative Research Team (in Science and Technology) in the University of Henan Province (no. 17IRTSTHN017).

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  1. College of Life Science, Henan Normal University, Xinxiang, 453007, Henan, People’s Republic of China

    Xiaohua Xia, Ruyan Wan, Weiran Huo, Linxia Zhang, Xiaopei Xia & Zhongjie Chang

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  1. Xiaohua Xia
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  2. Ruyan Wan
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Correspondence to Zhongjie Chang.

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Corresponding editor: Indrajit Nanda

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Xia, X., Wan, R., Huo, W. et al. Molecular cloning and mRNA expression pattern of \(\varvec{Sox}\)4 in Misgurnus anguillicaudatus. J Genet 97, 869–877 (2018). https://doi.org/10.1007/s12041-018-0972-2

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